在印度尼西亚日惹，使用重复的529 bp基因通过聚合酶链反应检测宠物猫粪便中的粪检患病率。
Detection of copro-prevalence by polymerase chain reaction using repetitive 529 bp gene in feces of pet cats () in Yogyakarta, Indonesia.
作者信息
Hanafiah Muhammad, Prastowo Joko, Hartati Sri, Aliza Dwinna, Nurcahyo Raden Wisnu
机构信息
Parasitology Laboratory, Faculty of Veterinary Medicine, Syiah Kuala University, Banda Aceh, Indonesia.
Department of Parasitology, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta, Indonesia.
出版信息
Vet World. 2018 Sep;11(9):1338-1343. doi: 10.14202/vetworld.2018.1338-1343. Epub 2018 Sep 28.
AIM
The aim of this research was to determine the copro-prevalence of using polymerase chain reaction (PCR) with repetitive 529 bp gene and to construct the phylogenetic tree of oocyst from pet cats in Yogyakarta.
MATERIALS AND METHODS
9 of 132 pet cat samples which serologically positive for were used in this research. To determine the copro-prevalence of in pet cat, 10 g of feces samples taken from practitioners and household cats in Yogyakarta were used in the PCR method utilizing repetitive 529 bp gene sequences.
RESULTS
The result shows that copro-prevalence by PCR using repetitive 529 bp gene was 33.3% (3/9). The phylogenetic tree of grouped into two clades, which clade 1 consists of isolates collected from pet cats in Yogyakarta Indonesia and isolates from China and in clade 2 consist of the isolates from India.
CONCLUSION
Copro-prevalence of in pet cats in Yogyakarta by means of PCR using repetitive 529 bp gene is around 33.3%.
目的
本研究旨在通过使用重复的529 bp基因的聚合酶链反应（PCR）来确定粪检患病率，并构建日惹市宠物猫卵囊的系统发育树。
材料与方法
本研究使用了132份血清学检测呈阳性的宠物猫样本中的9份。为了确定宠物猫中[具体寄生虫名称未给出]的粪检患病率，采用PCR方法，利用重复的529 bp基因序列，对取自日惹市执业兽医处和家猫的10克粪便样本进行检测。
结果
结果显示，使用重复的529 bp基因进行PCR检测的粪检患病率为33.3%（3/9）。[具体寄生虫名称未给出]的系统发育树分为两个分支，其中分支1由从印度尼西亚日惹市的宠物猫收集的[具体寄生虫名称未给出]分离株以及来自中国的[具体寄生虫名称未给出]分离株组成，分支2由来自印度的[具体寄生虫名称未给出]分离株组成。
结论
通过使用重复的529 bp基因的PCR方法，日惹市宠物猫中[具体寄生虫名称未给出]的粪检患病率约为33.3%。
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