Kemble G W, McCormick A L, Pereira L, Mocarski E S
J Virol. 1987 Oct;61(10):3143-51. doi: 10.1128/JVI.61.10.3143-3151.1987.
We demonstrated the presence of a single-stranded DNA-binding protein in human cytomegalovirus (CMV)-infected cells with properties analogous to those of herpes simplex virus (HSV) ICP8. Using monoclonal antibody specific for the CMV protein, we analyzed its fluorescence pattern and time of synthesis, mapped the gene encoding it by using a lambda gt11 library of CMV DNA fragments, and monitored its purification by phosphocellulose and DNA-Sepharose chromatography. In all characteristics we examined, the CMV protein behaved analogously to HSV ICP8. Our results are consistent with a functional role of CMV ICP8 in viral replication that is similar to that of HSV ICP8 and with the evolutionary conservation of the gene of interest in two divergent herpesviruses.
我们证实在人巨细胞病毒（CMV）感染的细胞中存在一种单链DNA结合蛋白，其特性类似于单纯疱疹病毒（HSV）的ICP8。利用针对CMV蛋白的单克隆抗体，我们分析了其荧光模式和合成时间，通过使用CMV DNA片段的λgt11文库对编码它的基因进行定位，并通过磷酸纤维素和DNA-琼脂糖凝胶色谱监测其纯化过程。在我们检测的所有特征中，CMV蛋白的表现与HSV ICP8类似。我们的结果与CMV ICP8在病毒复制中具有类似于HSV ICP8的功能作用以及两种不同疱疹病毒中相关基因的进化保守性相一致。
