Liu Xin, Liu Kaiqing, Shan Baosen, Wei Shengjuan, Li Dongfeng, Han Haiyin, Wei Wei, Chen Jie, Liu Honglin, Zhang Lifan
1College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, 210095 China.
2College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, 056038 China.
J Anim Sci Biotechnol. 2018 Nov 1;9:76. doi: 10.1186/s40104-018-0292-7. eCollection 2018.
Preadipocyte differentiation plays a critical role in subcutaneous fat deposition in pigs. However, the roles of different RNAs, such as messenger RNAs (mRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) in the differentiation process of subcutaneous preadipocytes, are still largely unclear. In the present study, a transcriptome analysis, including the analysis of mRNAs, lncRNAs, and circRNAs, during different differentiation stages, namely, day 0 (D0), day 2 (D2), day 4 (D4), and day 8 (D8), of subcutaneous preadipocytes from Chinese Erhualian pigs was performed.
A total of 1554, 470, 1344, 1777, and 676 differentially expressed (DE) mRNAs, 112, 58, 95, 136, and 93 DE lncRNAs, and 902, 787, 710, 932, and 850 DE circRNAs were identified between D2 and D0, between D4 and D2, between D8 and D4, between D4 and D0, and between D8 and D0, respectively. Furthermore, functional enrichment analysis showed that the common DE mRNAs during the entire differentiation process were mainly involved in lipid metabolic and cell differentiation processes. Additionally, co-expression network analysis identified the potential lncRNAs related to adipogenesis, e.g., MSTRG.131380 and MSTRG.62128.
Our study provides new insights of the expression changes of RNAs during adipogenic differentiation, which might contribute to the phenotype of subcutaneous adipogenesis. These results greatly improve our understanding of the molecular mechanisms regulating subcutaneous fat deposition in pigs.
前体脂肪细胞分化在猪的皮下脂肪沉积中起着关键作用。然而，不同类型的RNA，如信使RNA（mRNA）、长链非编码RNA（lncRNA）和环状RNA（circRNA）在皮下前体脂肪细胞分化过程中的作用仍不清楚。在本研究中，对中国二花脸猪皮下前体脂肪细胞在不同分化阶段，即第0天（D0）、第2天（D2）、第4天（D4）和第8天（D8）进行了转录组分析，包括mRNA、lncRNA和circRNA的分析。
在D2和D0之间、D4和D2之间、D8和D4之间、D4和D0之间以及D8和D0之间，分别鉴定出1554、470、1344、1777和676个差异表达（DE）mRNA，112、58、95、136和93个DE lncRNA，以及902、787、710、932和850个DE circRNA。此外，功能富集分析表明，整个分化过程中常见的DE mRNA主要参与脂质代谢和细胞分化过程。此外，共表达网络分析确定了与脂肪生成相关的潜在lncRNA，例如MSTRG.131380和MSTRG.62128。
我们的研究为脂肪生成分化过程中RNA的表达变化提供了新的见解，这可能有助于皮下脂肪生成的表型。这些结果极大地提高了我们对猪皮下脂肪沉积调控分子机制的理解。
