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猪肌肉内脂肪的 lncRNA-miRNA-mRNA 调控网络的综合分析。

Comprehensive Analysis of the lncRNA-miRNA-mRNA Regulatory Network for Intramuscular Fat in Pigs.

机构信息

Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China.

Beijing Animal Husbandry Station, Beijing 100101, China.

出版信息

Genes (Basel). 2023 Jan 7;14(1):168. doi: 10.3390/genes14010168.

DOI:10.3390/genes14010168
PMID:36672909
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9859044/
Abstract

Intramuscular fat (IMF) is an essential trait closely related to meat quality. The IMF trait is a complex quantitative trait that is regulated by multiple genes. In order to better understand the process of IMF and explore the key factors affecting IMF deposition, we identified differentially expressed mRNA, miRNA, and lncRNA in the muscle (LD) between Songliao Black (SL) pigs and Landrace pigs. We obtained 606 differentially expressed genes (DEGs), 55 differentially expressed miRNAs (DEMs), and 30 differentially expressed lncRNAs (DELs) between the SL pig and Landrace pig. Enrichment results from GO and KEGG indicate that DEGs are involved in fatty acid metabolism and some pathways related to glycogen synthesis. We constructed an lncRNA-miRNA-mRNA interaction network with 18 DELs, 11 DEMs, and 42 DEGs. Finally, the research suggests that , , , , , , , , , and may be the key genes affecting IMF deposition. The LncRNAs MSTRG.19948.1, MSTRG.13120.1, MSTRG.20210.1, and MSTRG.10023.1, and the miRNAs ssc-miRNA-429 and ssc-miRNA-7-1, may play a regulatory role in IMF deposition through their respective target genes. Our research provides a reference for further understanding the regulatory mechanism of IMF.

摘要

肌内脂肪（IMF）是与肉质密切相关的重要特征。IMF 性状是一个复杂的数量性状，受多个基因调控。为了更好地了解 IMF 的形成过程，探索影响 IMF 沉积的关键因素，我们在松辽黑猪和长白猪的肌肉（LD）中鉴定了差异表达的 mRNA、miRNA 和 lncRNA。我们在松辽黑猪和长白猪之间获得了 606 个差异表达基因（DEGs）、55 个差异表达 miRNA（DEMs）和 30 个差异表达 lncRNA（DELs）。GO 和 KEGG 的富集结果表明，DEGs 参与脂肪酸代谢和一些与糖原合成相关的途径。我们构建了一个包含 18 个 DELs、11 个 DEMs 和 42 个 DEGs 的 lncRNA-miRNA-mRNA 相互作用网络。最后，研究表明， 、 、 、 、 、 、 、 和 可能是影响 IMF 沉积的关键基因。LncRNAs MSTRG.19948.1、MSTRG.13120.1、MSTRG.20210.1 和 MSTRG.10023.1 以及 miRNAs ssc-miRNA-429 和 ssc-miRNA-7-1 可能通过各自的靶基因在 IMF 沉积中发挥调节作用。我们的研究为进一步了解 IMF 的调控机制提供了参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/adbaeb9a884f/genes-14-00168-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/6b01208ed2a2/genes-14-00168-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/d425c4021374/genes-14-00168-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/a46bd9143f03/genes-14-00168-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/188a9e5a5d9e/genes-14-00168-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/9ce97e8209e2/genes-14-00168-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/adbaeb9a884f/genes-14-00168-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/6b01208ed2a2/genes-14-00168-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/d425c4021374/genes-14-00168-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/a46bd9143f03/genes-14-00168-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/188a9e5a5d9e/genes-14-00168-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/9ce97e8209e2/genes-14-00168-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/72c9/9859044/adbaeb9a884f/genes-14-00168-g006.jpg

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