State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics , Chinese Academy of Sciences , Shanghai 200050 , China.
Center of Materials Science and Optoelectronics Engineering , University of Chinese Academy of Science , Beijing 100049 , China.
ACS Appl Mater Interfaces. 2018 Dec 12;10(49):42018-42029. doi: 10.1021/acsami.8b15724. Epub 2018 Nov 27.
The interactions between material surfaces and bacteria/cells have been widely investigated, based on which biomaterials with antibacterial and osteogenic abilities can be designed to conquer implant failures. The pH of environments is known to affect bacterial growth and bone formation/resorption, and it is possible that the antibacterial and osteogenic abilities of biomaterials can be simultaneously improved by regulating their surface alkalinity. Herein, we fabricated many kinds of films with various alkalinity levels on titanium surface to explore the effect of local alkaline microenvironments around material surfaces on the behaviors of bacteria and osteogenic cells. Both Gram-positive and -negative bacteria were cultured on sample surfaces to investigate their antibacterial effects. Cell adhesion, proliferation, and alkaline phosphatase (ALP) activities were investigated by culturing both bone mesenchymal stem cells (MSCs) and osteoblast cells on sample surfaces. The results show that an appropriate local alkaline environment can effectively inhibit the growth of both Gram-positive and -negative bacteria through inactivating ATP synthesis and inducing oxidative stress. Meanwhile, it can promote the osteogenic differentiation of bone MSCs and enhance the proliferation and ALP activities of osteoblast cells. In conclusion, material surfaces endowed with appropriate alkalinity can possess antibacterial and osteogenic properties, which provide a novel strategy to design multifunctional biomaterials for bone generation.
材料表面与细菌/细胞的相互作用已得到广泛研究，在此基础上，可以设计具有抗菌和促成骨能力的生物材料来克服植入物失效的问题。环境的 pH 值已知会影响细菌的生长和骨的形成/吸收，并且有可能通过调节材料表面的碱性来同时提高生物材料的抗菌和促成骨能力。在此，我们在钛表面制备了多种具有不同碱度水平的薄膜，以探索材料表面周围局部碱性微环境对细菌和成骨细胞行为的影响。将革兰氏阳性菌和革兰氏阴性菌都培养在样品表面上，以研究其抗菌效果。通过在样品表面培养骨髓间充质干细胞（MSCs）和成骨细胞来研究细胞黏附、增殖和碱性磷酸酶（ALP）活性。结果表明，适当的局部碱性环境可以通过灭活 ATP 合成和诱导氧化应激来有效抑制革兰氏阳性菌和革兰氏阴性菌的生长。同时，它可以促进骨髓间充质干细胞的成骨分化，并增强成骨细胞的增殖和 ALP 活性。总之，具有适当碱度的材料表面可以具有抗菌和成骨特性，为设计用于骨生成的多功能生物材料提供了一种新策略。
