Huberman J A, Zhu J G, Davis L R, Newlon C S
Department of Molecular and Cellular Biology, Roswell Park Memorial Institute, Buffalo, NY 14263.
Nucleic Acids Res. 1988 Jul 25;16(14A):6373-84. doi: 10.1093/nar/16.14.6373.
Two dimensional gel electrophoretic techniques were used to locate all functional DNA replication origins in a 22.5 kb stretch of yeast chromosome III. Only one origin was detected, and that origin is located within several hundred bp of an ARS element.
二维凝胶电泳技术被用于定位酵母三号染色体22.5 kb片段中的所有功能性DNA复制起点。仅检测到一个起点,且该起点位于一个自主复制序列(ARS)元件的几百个碱基对范围内。