Biswas E E, Biswas S B
Division of Endocrinology, University of Maryland School of Medicine, Baltimore 21201.
Nucleic Acids Res. 1988 Jul 25;16(14A):6411-26. doi: 10.1093/nar/16.14.6411.
A partially purified primase-polymerase complex from the yeast, Saccharomyces cerevisiae, was capable of replicating a single stranded circular phage DNA into a replicative form with high efficiency. The primase-polymerase complex exhibited primase activity and polymerase activity on singly primed circular ssDNA as well as on gapped DNA. In addition, it was able to replicate an unprimed, single-stranded, circular phage DNA through a coupled primase-polymerase action. On Biogel A-O.5m filtration the primase-polymerase activities appeared in the void volume, demonstrating a mass of greater than 500 kilodaltons. Primase and various primase-polymerase complexes synthesized unique primers on single stranded DNA templates and the size distribution of primers was dependent on the structure of the DNA and the nature of the primase-polymerase assembly.
从酿酒酵母中提取的部分纯化的引发酶 - 聚合酶复合物能够高效地将单链环状噬菌体DNA复制成复制形式。该引发酶 - 聚合酶复合物在单引物环状单链DNA以及缺口DNA上均表现出引发酶活性和聚合酶活性。此外，它能够通过引发酶 - 聚合酶的偶联作用复制无引物的单链环状噬菌体DNA。在Biogel A - 0.5m过滤中，引发酶 - 聚合酶活性出现在空体积中，表明其质量大于500千道尔顿。引发酶和各种引发酶 - 聚合酶复合物在单链DNA模板上合成独特的引物，引物的大小分布取决于DNA的结构和引发酶 - 聚合酶组装的性质。
