Singh H, Dumas L B
J Biol Chem. 1984 Jun 25;259(12):7936-40.
Biochemical fractionation of the yeast Saccharomyces cerevisiae has revealed a novel DNA primase activity that copurifies with the major DNA polymerase activity. In the presence of RNA precursors and single-stranded DNA (poly(dT), M13), the DNA primase synthesizes discrete length oligoribonucleotides (apparent length, 8-12 nucleotides) as well as longer RNA chains that appear to be multiples of a modal length of 11-12 nucleotides. When DNA precursors are also present, the oligoribonucleotides are utilized by the accompanying DNA polymerase as primers for DNA synthesis. Copurification of these two enzymatic activities suggests their association in a physical complex which may function in the synthesis of Okazaki fragments at chromosomal replication forks.
对酿酒酵母进行生化分级分离，揭示了一种新的DNA引发酶活性，它与主要的DNA聚合酶活性共同纯化。在RNA前体和单链DNA（聚（dT）、M13）存在的情况下，DNA引发酶合成离散长度的寡核糖核苷酸（表观长度为8 - 12个核苷酸）以及更长的RNA链，这些RNA链似乎是11 - 12个核苷酸的模式长度的倍数。当也存在DNA前体时，寡核糖核苷酸被伴随的DNA聚合酶用作DNA合成的引物。这两种酶活性的共同纯化表明它们在一个物理复合物中结合，该复合物可能在染色体复制叉处冈崎片段的合成中发挥作用。
