Corso Maira N, Marques Lis S, Gracia Luis F G, Rodrigues Rômulo B, Barcellos Leonardo J G, Streit Danilo P
Programa de Pós-Graduação em Zootecnia, Universidade Federal do Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil.
Programa de Pós-Graduação em Zootecnia, Universidade Federal do Rio Grande do Sul, 91540-000, Porto Alegre, RS, Brazil.
Theriogenology. 2019 Feb;125:135-139. doi: 10.1016/j.theriogenology.2018.10.033. Epub 2018 Oct 31.
The production of captive fish is only possible through artificial reproduction, but manipulation is a known stressor stimulus. Thus, the objective of the present study was to evaluate the effects of different eugenol concentrations (0, 30, 40, 50 and 60 mg/L) during reproductive management of Rhamdia quelen. Seventy-five mature male R. quelen were randomly distributed among the five treatments, and blood samples were collected at the time of semen collection to measure plasma cortisol. The following parameters were evaluated in the fresh semen samples: motility, motility duration, concentration and fertilization rate. The following parameters were evaluated in the frozen semen samples: motility, motility duration, morphology, membrane integrity, DNA integrity and mitochondrial functionality. The animals anesthetized with eugenol at concentrations of 40 and 50 mg/L had lower levels of plasma cortisol (88.4 and 83.3 ng/mL, respectively) than the control (147.1 ng/mL). For fresh semen, the control treatment presented the highest rate and time of motility but differed (P < 0.05) only from the animals treated with 60 mg/L eugenol. For the cryopreserved semen the highest rates and motility time were observed in the control treatment and in the animals anesthetized with 40 mg/L eugenol, differing (P < 0.05) from anesthetized animals with 50 and 60 mg/L. Mitochondrial functionality was higher in fish anesthetized with 30 mg/L eugenol differing only for animals anesthetized with 60 mg/L. There was no difference between treatments for sperm concentration and fertilization rate of fresh semen. There were no differences (P > 0.05) between treatments in the parameters of membrane integrity, DNA integrity and% of normal spermatozoa after thawing of the cryopreserved semen samples. The use of 30, 40 and 50 mg/L eugenol maintained the seminal quality of the fresh semen, and the quality of the thawed semen was maintained with 30 and 40 mg/L eugenol. These results show that stress reduction can be reconciled with reproductive management without compromising reproductive performance.
圈养鱼的生产只能通过人工繁殖来实现，但操作是一种已知的应激源刺激。因此，本研究的目的是评估不同丁香酚浓度（0、30、40、50和60mg/L）对奎氏溪蟾生殖管理的影响。75只成熟雄性奎氏溪蟾被随机分配到五种处理中，并在采集精液时采集血样以测量血浆皮质醇。对新鲜精液样本评估以下参数：活力、活力持续时间、浓度和受精率。对冷冻精液样本评估以下参数：活力、活力持续时间、形态、膜完整性、DNA完整性和线粒体功能。用40和50mg/L丁香酚麻醉的动物血浆皮质醇水平（分别为88.4和83.3ng/mL）低于对照组（147.1ng/mL）。对于新鲜精液，对照处理的活力率和活力时间最高，但仅与用60mg/L丁香酚处理的动物不同（P<0.05）。对于冷冻精液，对照处理和用40mg/L丁香酚麻醉的动物观察到最高的活力率和活力时间，与用50和60mg/L麻醉的动物不同（P<0.05）。用30mg/L丁香酚麻醉的鱼线粒体功能较高，仅与用60mg/L麻醉的动物不同。新鲜精液的精子浓度和受精率在各处理之间没有差异。冷冻精液样本解冻后的膜完整性、DNA完整性和正常精子百分比参数在各处理之间没有差异（P>0.05）。使用30、40和50mg/L丁香酚可保持新鲜精液的精液质量，使用30和40mg/L丁香酚可保持解冻后精液的质量。这些结果表明，在不影响繁殖性能的情况下，减轻应激与生殖管理可以兼顾。
