Teixeira Nathalia Dos Santos, Marques Lis Santos, Rodrigues Rômulo Batista, Gusso Darlan, Pinheiro Gabriela Thais, Machado Thales Lysakowski Flores, Streit Danilo Pedro
Animal Science Research Program of Federal University of Rio Grande Do Sul, Porto Alegre, RS, Brazil.
Postgraduate Program in Cellular and Molecular Biology, Neurochemistry and Psychopharmacology Laboratory, School of Sciences, Pontifical Catholic University of Rio Grande Do Sul, Porto Alegre, RS, Brazil.
Anim Reprod Sci. 2021 Feb;225:106669. doi: 10.1016/j.anireprosci.2020.106669. Epub 2020 Dec 3.
Anesthesia is a common practice used in fish research and aquaculture. It is important to understand anesthetic effects on the animal and tissues of interest to ensure validity of data and to improve animal welfare in research and fish production endeavors. The production of some captive fish species is only possible by imposing artificial reproduction procedures, and manipulation of fish for these purposes is a stressor. The purpose of this study, therefore, was to evaluate effects of different concentrations (100, 200, and 300 mg/L) of the anesthetic MS-222 (tricaine methanesulfonate) on cortisol concentrations and effects on sperm quality in Rhamdia quelen. After hormonal induction of gamete production, 28 sexually mature males were randomly assigned to treatments, and milt and blood samples were collected. Anesthesia induction time, motility rate, sperm concentration and morphology, plasma cortisol concentrations, and reproductive hormone concentrations (testosterone, 17-α-hydroxyprogesterone, and estradiol) were evaluated. Sperm motility was greater in the control than 300 mg/L treatment group but did not differ among the control, 100, and 200 mg/L groups. The estradiol concentration was greater in non- anesthetized than anesthetized Rhamdia quelen, but plasma cortisol concentrations did not differ among treatment groups (182.50 ± 42.03 ng/mL). The anesthetic MS-222 at concentrations of 100, 200, and 300 mg/L did not inhibit the stress response due to handling of Rhamdia quelen males. In addition, treatment with MS-222 was not effective in inhibiting detrimental effects on sperm quality because this treatment was associated with impaired sperm motility and lesser concentrations of plasma estradiol.
麻醉是鱼类研究和水产养殖中常用的操作。了解麻醉对目标动物和组织的影响对于确保数据的有效性以及提高研究和鱼类生产活动中的动物福利至关重要。一些圈养鱼类品种只有通过人工繁殖程序才能进行生产，而为实现这些目的对鱼类进行的操作是一种应激源。因此，本研究的目的是评估不同浓度（100、200和300mg/L）的麻醉剂MS-222（三卡因甲磺酸盐）对奎氏溪鳉皮质醇浓度的影响以及对精子质量的影响。在通过激素诱导配子产生后，将28只性成熟雄性随机分配到各处理组，并采集精液和血液样本。评估麻醉诱导时间、活力率、精子浓度和形态、血浆皮质醇浓度以及生殖激素浓度（睾酮、17-α-羟基孕酮和雌二醇）。对照组的精子活力高于300mg/L处理组，但对照组、100mg/L和200mg/L组之间无差异。未麻醉的奎氏溪鳉的雌二醇浓度高于麻醉的，但各处理组的血浆皮质醇浓度无差异（182.50±42.03ng/mL）。浓度为100、200和300mg/L的麻醉剂MS-222并未抑制奎氏溪鳉雄性因处理而产生的应激反应。此外，用MS-222处理在抑制对精子质量的有害影响方面无效，因为这种处理与精子活力受损和血浆雌二醇浓度降低有关。
