Universidade Regional Integrada do Alto Uruguai e das Missões, Erechim, Rio Grande do Sul, Brazil.
Universidade do Extremo Sul Catarinense, Criciúma, Santa Catarina, Brazil.
Arch Biochem Biophys. 2019 Jan;661:50-55. doi: 10.1016/j.abb.2018.11.003. Epub 2018 Nov 8.
The present study sought to evaluate the effects of physical training on histological parameters and oxidative stress in the myocardium of mice chronically exposed to hand-rolled cornhusk cigarette (HRCC) smoke. Male Swiss mice (60 days old, 30-35 g) were either exposed to ambient air or passively exposed to the smoke of 12 cigarettes daily over 3 sessions (4 cigarettes per session) for 60 consecutive days with or without physical training for 8 weeks. Forty-eight hours after the last training session, the heart was surgically removed for histological analysis and measurement of oxidative stress parameters. Histological imaging revealed cell disruption, with poorly defined nuclei, in the mice exposed to HRCC smoke, but not in the control group. However, mice exposed to HRCC smoke with physical training displayed signs of tissue repair and improved tissue integrity. Biochemical analysis revealed decreased production of superoxide, 2',7'-dichlorofluorescein (DCF), and nitrite, as well as decreased protein carbonylation, in the physical training groups, likely due to the exercise-induced increase in glutathione peroxidase (GPX) activity and glutathione (GSH) content. Taken together, our results suggest that physical exercise exerts cardioprotective effects by modulating the redox responses in animals exposed to HRCC smoke.
本研究旨在评估体力训练对长期暴露于手工卷玉米皮香烟（HRCC）烟雾的小鼠心肌组织学参数和氧化应激的影响。雄性瑞士小鼠（60 天大，30-35g）被暴露于环境空气中或被动暴露于每日 3 次（每次 4 支香烟）的 HRCC 烟雾中，持续 60 天，同时或不进行 8 周的体力训练。最后一次训练后 48 小时，手术取出心脏进行组织学分析和氧化应激参数测量。组织学成像显示，暴露于 HRCC 烟雾的小鼠出现了细胞破裂，细胞核定义不清晰，但在对照组中则没有这种情况。然而，暴露于 HRCC 烟雾并进行体力训练的小鼠显示出组织修复的迹象，组织完整性得到改善。生化分析显示，体力训练组中超氧化物、2'，7'-二氯荧光素（DCF）和亚硝酸盐的产生减少，蛋白质羰基化减少，这可能是由于运动诱导的谷胱甘肽过氧化物酶（GPX）活性和谷胱甘肽（GSH）含量增加所致。综上所述，我们的结果表明，体力训练通过调节暴露于 HRCC 烟雾的动物的氧化还原反应发挥心脏保护作用。
