Centre de recherche du Centre hospitalier universitaire (CHU) de Québec, Université Laval, axe médecine régénératrice, Hôpital du Saint-Sacrement, Québec, QC, Canada; Centre de recherche en organogénèse expérimentale de l'Université Laval / LOEX, Québec, QC, Canada; Département d'Ophtalmologie et d'oto-rhino-laryngologie-chirurgie cervico-faciale, Faculté de médecine, Université Laval, Québec, QC, Canada.
Centre de recherche de l'Hôpital Maisonneuve-Rosemont (HMR) and Département d'ophtalmologie, Université de Montréal, Montréal, QC, Canada.
Exp Eye Res. 2019 Feb;179:102-105. doi: 10.1016/j.exer.2018.11.004. Epub 2018 Nov 8.
Cells and tissues are influenced by environmental conditions. In vivo, the corneal endothelium is subjected to hydrostatic intraocular pressure (IOP) and to the hydrokinetic pressure of the moving aqueous humor in the anterior chamber. In this paper, we used a corneal bioreactor to recreate the IOP condition and investigated the effect of the in vivo hydrodynamic environment of corneal endothelial cells on the formation of tight junctions. Native ex vivo corneas and engineered corneal endothelia subjected to pressure showed an increase in ZO-1 expression at the cell periphery. Pressure also improved the corneal transparency of engineered and native corneas. Corneal thickness was accordingly reduced from 926 ± 70 μm to 651 ± 70 μm for the engineered corneal endothelium and from 847 ± 27 μm to 571 ± 23 μm for the native endothelium. These results suggest that the hydrodynamic pressure of the anterior chamber is important for the cell junction integrity of the corneal endothelium.
细胞和组织受到环境条件的影响。在体内，角膜内皮细胞受到眼内静水压力 (IOP) 和前房内流动房水的动压力的影响。在本文中，我们使用角膜生物反应器来重现 IOP 条件，并研究了角膜内皮细胞的体内流体动力学环境对紧密连接形成的影响。在体外培养的天然角膜和经压力处理的工程化角膜内皮细胞中，细胞边缘的 ZO-1 表达增加。压力还提高了工程化和天然角膜的透明度。相应地，工程化角膜内皮的角膜厚度从 926±70μm 减少到 651±70μm，天然内皮的角膜厚度从 847±27μm 减少到 571±23μm。这些结果表明，前房的流体动力压力对于角膜内皮细胞连接的完整性很重要。
