Duan Sujuan, Li Yingjie, Zhang Yanyan, Zhu Xuan, Mei Yan, Xu Dongmei, Huang Guofu
Department of Ophthalmology, First Hospital of Nanchang, Nanchang, Jiangxi 330008, China.
Jiangxi Key Laboratory of Cancer Metastasis and Precision Treatment, First Hospital of Nanchang, Nanchang, Jiangxi 330008, China.
J Ophthalmol. 2021 Nov 27;2021:9217866. doi: 10.1155/2021/9217866. eCollection 2021.
Corneal endothelial cells are usually exposed to shear stress caused by the aqueous humour, which is similar to the exposure of vascular endothelial cells to shear stress caused by blood flow. However, the effect of fluid shear stress on corneal endothelial cells is still poorly understood. The purpose of this study was to explore whether the shear stress that results from the aqueous humour influences corneal endothelial cells.
An in vitro model was established to generate fluid flow on cells, and the effect of fluid flow on corneal endothelial cells after exposure to two levels of shear stress for different durations was investigated. The mRNA and protein expression of corneal endothelium-related markers in rabbit corneal endothelial cells was evaluated by real-time PCR and western blotting.
The expression of the corneal endothelium-related markers ZO-1, N-cadherin, and Na-K-ATPase in rabbit corneal endothelial cells (RCECs) was upregulated at both the mRNA and protein levels after exposure to shear stress.
This study demonstrates that RCECs respond favourably to fluid shear stress, which may contribute to the maintenance of corneal endothelial cell function. Furthermore, this study also provides a theoretical foundation for further investigating the response of human corneal endothelial cells to the shear stress caused by the aqueous humour.
角膜内皮细胞通常暴露于房水引起的剪切应力下，这类似于血管内皮细胞暴露于血流引起的剪切应力。然而，流体剪切应力对角膜内皮细胞的影响仍知之甚少。本研究的目的是探讨房水产生的剪切应力是否会影响角膜内皮细胞。
建立体外模型以在细胞上产生流体流动，并研究在暴露于两种水平的剪切应力不同持续时间后流体流动对角膜内皮细胞的影响。通过实时PCR和蛋白质印迹法评估兔角膜内皮细胞中角膜内皮相关标志物的mRNA和蛋白质表达。
暴露于剪切应力后，兔角膜内皮细胞（RCECs）中角膜内皮相关标志物ZO-1、N-钙黏蛋白和钠钾ATP酶的表达在mRNA和蛋白质水平均上调。
本研究表明RCECs对流体剪切应力反应良好，这可能有助于维持角膜内皮细胞功能。此外，本研究还为进一步研究人角膜内皮细胞对房水引起的剪切应力的反应提供了理论基础。
