Chemical Engineering & Pharmaceutical College, Henan Univ. of Science and Technology, 263 Kai Yuan Road, Luolong District 471023, Luoyang, P.R. China.
J Food Sci. 2018 Dec;83(12):2943-2953. doi: 10.1111/1750-3841.14378. Epub 2018 Nov 11.
Ultrasound-assisted extraction (UAE) of total monoterpene glycosides extract (TMGE) from oil peony seed cakes was investigated. The extraction yield was optimized using response surface methodology (RSM). The chemical constituents of the monoterpene glycosides extract were isolated by repeated column chromatography, and the contents of the main isolated monoterpene glycosides in the oil peony seed cakes were determined by HPLC. The optimum conditions were as follows: a liquid-to-solid ratio of 27 mL/g, ultrasonic extraction time of 16 min, ultrasonic extraction temperature of 26 °C, and ethanol concentration of 67%. Under these conditions, the extraction yield of TMGE was 10.24%. Twenty monoterpene glycosides were isolated from the oil peony seed cakes, and compounds 11-12, 16 and 20 showed strong inhibitory activities on NO production. TMGE from oil peony seed cakes can also to be used as promising immunosuppressive drug due to its high content of monoterpene glycosides and immune-inhibitory activity. PRACTICAL AAPPLICATION: The peony seed oil was authorized as a new food by the Ministry of Health of the People's Republic of China. Peony seed cake is one of the most important by-products in the preparation of peony seed oil, and accounts for approximately 40% of the total mass of the peony seed. Total monoterpene glycosides are the main active ingredient of oil peony seed cake. This research has optimized the extraction conditions of total monoterpene glycoside from seeds cake of Paeonia ostii, which will provide useful reference information for further studies, and offer related industries with helpful guidance in practice.
超声辅助提取（UAE）牡丹籽饼粕中总单萜苷提取物（TMGE）的工艺条件进行了研究。采用响应面法（RSM）对提取工艺进行优化。通过反复柱层析分离单萜苷提取物的化学成分，并采用 HPLC 法测定牡丹籽饼粕中单萜苷的含量。最佳条件为：液料比 27 mL/g，超声提取时间 16 min，超声提取温度 26℃，乙醇浓度 67%。在此条件下，TMGE 的提取率为 10.24%。从牡丹籽饼粕中分离得到 20 种单萜苷类化合物，其中化合物 11-12、16 和 20 对 NO 生成具有较强的抑制活性。牡丹籽饼粕中的 TMGE 由于其含有丰富的单萜苷类化合物和免疫抑制活性，可作为有前途的免疫抑制剂药物。实际应用：牡丹籽油已被中华人民共和国卫生部批准为新食品。牡丹籽饼是牡丹籽油制备过程中最重要的副产物之一，占牡丹籽总质量的 40%左右。总单萜苷是牡丹籽饼的主要活性成分。本研究优化了牡丹籽油籽饼中单萜苷的提取条件，为进一步研究提供了有用的参考信息，并为相关行业的实践提供了有益的指导。
