Chemical Engineering & Pharmaceutical College, Henan University of Science and Technology, Luoyang, China.
Phytochem Anal. 2019 Jul;30(4):464-473. doi: 10.1002/pca.2829. Epub 2019 Apr 4.
The Paeonia ostii T. Hong & J. X. Zhang seed shell, characterised by a high content of oligostilbenes, is one of the two most important by-products in the preparation of seed oil. Oligostilbenes are considered characteristic constituents of the genus Paeonia, and can be used in fingerprinting to determine the geographical origin and the quality of raw materials.
To develop and optimise a simple and reproducible high-performance liquid chromatography diode array detection (HPLC-DAD) method for the simultaneous determination of seven oligostilbenes in P. ostii seed shell from different geographical areas, and to associate the cultivation area.
A validated HPLC method coupled with a DAD detector was performed for the detection and determination of target compounds in the samples. Optimal chromatographic conditions were achieved using an Agilent Zorbax Eclipse SB-AQ-C18 column and a gradient elution with acetonitrile and potassium dihydrogen phosphate solution.
The proposed quantitative method showed appropriate accuracy and precision, and was successfully applied to the routine analysis of seven oligostilbenes and the quality evaluation of 50 P. ostii seed shell samples. There were significant differences between the contents of the seven oligostilbenes in different samples (P < 0.01).
The results demonstrated that the oligostilbenes were main secondary metabolites in the P. ostii seed shells, and the content of seven components in P. ostii seed shells sourced from different cultivation areas in China was different.
牡丹 Ostii T. Hong 种子壳的特征是含有高浓度的低聚苯乙烯，是制备种子油的两个最重要的副产物之一。低聚苯乙烯被认为是牡丹属的特征成分，可用于指纹图谱，以确定原料的地理来源和质量。
开发和优化一种简单且可重现的高效液相色谱二极管阵列检测（HPLC-DAD）方法，用于同时测定来自不同地理区域的牡丹 Ostii 种子壳中的七种低聚苯乙烯，并与种植区相关联。
采用经过验证的 HPLC 方法与 DAD 检测器相结合，对样品中的目标化合物进行检测和测定。采用 Agilent Zorbax Eclipse SB-AQ-C18 柱，以乙腈和磷酸二氢钾溶液梯度洗脱，优化了色谱条件。
所提出的定量方法具有适当的准确性和精密度，并成功应用于七种低聚苯乙烯的常规分析和 50 个牡丹 Ostii 种子壳样品的质量评价。不同样品中七种低聚苯乙烯的含量存在显著差异（P<0.01）。
结果表明，低聚苯乙烯是牡丹 Ostii 种子壳中的主要次生代谢物，来自中国不同种植区的牡丹 Ostii 种子壳中七种成分的含量不同。
