Lee Ayeong, Kim Ji Yea, Heo Jina, Cho Dae-Hyun, Kim Hee-Sik, An In-Sook, An Sungkwan, Bae Seunghee
Research Institute for Molecular-Targeted Drugs, Department of Cosmetics Engineering, Konkuk University, Seoul 05029, Republic of Korea.
Korea Institute for Skin and Clinical Sciences, Gene Cell Pharm Corporation, Seoul 05029, Republic of Korea.
J Microbiol Biotechnol. 2018 Dec 28;28(12):2121-2132. doi: 10.4014/jmb.1810.10008.
Abnormal melanin synthesis results in several hyperpigmentary disorders such as freckles, melanoderma, age spots, and other related conditions. In this study, we investigated the antimelanogenic effects of an extract from the microalgae (CE) and potential mechanisms responsible for its inhibitory effect in B16F10, normal human epidermal melanocyte cells, and human skin-equivalent models. The CE extract showed significant dose-dependent inhibitory effects on α-melanocyte-stimulating, hormone-induced melanin synthesis in cells. Additionally, the CE extract exhibited suppressive effects on the mRNA and protein expression of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein-1, and tyrosinase-related protein-2. The CE extract also inhibited the phosphorylation of protein kinase A and extracellular signal-related kinase, which function as upstream regulators of melanogenesis. Using a three-dimensional, reconstructed pigmented epidermis model, the CE-mediated, anti-pigmentation effects were confirmed by Fontana-Masson staining and melanin content assays. Taken together, CE extract can be used as an anti-pigmentation agent.
异常的黑色素合成会导致多种色素沉着过度性疾病，如雀斑、黑皮病、老年斑及其他相关病症。在本研究中，我们调查了微藻提取物（CE）的抗黑色素生成作用及其在B16F10细胞、正常人表皮黑素细胞和人皮肤等效模型中发挥抑制作用的潜在机制。CE提取物对α-黑素细胞刺激激素诱导的细胞内黑色素合成表现出显著的剂量依赖性抑制作用。此外，CE提取物对小眼畸形相关转录因子、酪氨酸酶、酪氨酸酶相关蛋白-1和酪氨酸酶相关蛋白-2的mRNA和蛋白表达具有抑制作用。CE提取物还抑制了蛋白激酶A和细胞外信号调节激酶的磷酸化，这两种激酶是黑色素生成的上游调节因子。使用三维重建的色素沉着表皮模型，通过Fontana-Masson染色和黑色素含量测定证实了CE介导的抗色素沉着作用。综上所述，CE提取物可作为一种抗色素沉着剂。
