Cho Juhee, Bejaoui Meriem, Tominaga Kenichi, Isoda Hiroko
Alliance for Research on the Mediterranean and North Africa (ARENA), University of Tsukuba, Tsukuba 305-0006, Japan.
Open Innovation Laboratory for Food and Medicinal Resource Engineering, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba 305-8577, Japan.
Int J Mol Sci. 2024 Apr 19;25(8):4479. doi: 10.3390/ijms25084479.
Olive leaf contains plenty of phenolic compounds, among which oleuropein (OP) is the main component and belongs to the group of secoiridoids. Additionally, phenolic compounds such as oleocanthal (OL) and oleacein (OC), which share a structural similarity with OP and two aldehyde groups, are also present in olive leaves. These compounds have been studied for several health benefits, such as anti-cancer and antioxidant effects. However, their impact on the skin remains unknown. Therefore, this study aims to compare the effects of these three compounds on melanogenesis using B16F10 cells and human epidermal cells. Thousands of gene expressions were measured by global gene expression profiling with B16F10 cells. We found that glutaraldehyde compounds derived from olive leaves have a potential effect on the activation of the melanogenesis pathway and inducing differentiation in B16F10 cells. Accordingly, the pro-melanogenesis effect was investigated by means of melanin quantification, mRNA, and protein expression using human epidermal melanocytes (HEM). This study suggests that secoiridoid and its derivates have an impact on skin protection by promoting melanin production in both human and mouse cell lines.
橄榄叶含有大量酚类化合物，其中橄榄苦苷（OP）是主要成分，属于裂环环烯醚萜类。此外，橄榄叶中还存在与OP结构相似且含有两个醛基的酚类化合物，如油橄榄苦醛（OL）和橄榄素（OC）。这些化合物已被研究具有多种健康益处，如抗癌和抗氧化作用。然而，它们对皮肤的影响仍然未知。因此，本研究旨在使用B16F10细胞和人表皮细胞比较这三种化合物对黑色素生成的影响。通过对B16F10细胞进行全基因表达谱分析，测量了数千个基因的表达。我们发现橄榄叶衍生的戊二醛化合物对B16F10细胞中黑色素生成途径的激活和诱导分化具有潜在作用。因此，使用人表皮黑素细胞（HEM）通过黑色素定量、mRNA和蛋白质表达来研究促黑色素生成作用。本研究表明，裂环环烯醚萜及其衍生物通过促进人和小鼠细胞系中的黑色素生成对皮肤保护产生影响。
