Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama, 930-8555, Japan; Graduate School of Innovative Life Science, University of Toyama, Gofuku 3190, Toyama, 930-8555, Japan.
Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama, 930-8555, Japan; Graduate School of Innovative Life Science, University of Toyama, Gofuku 3190, Toyama, 930-8555, Japan.
Biochem Biophys Res Commun. 2018 Dec 9;507(1-4):136-141. doi: 10.1016/j.bbrc.2018.10.188. Epub 2018 Nov 8.
In the RNA world, enrichment of self-replicating RNAs would have been beneficial to their survival, amplification, and evolution. Self-assembly of RNAs may be a strategy by which they enrich themselves. We examined the effects of molecular crowding on the activity of a bimolecular group I ribozyme and its derivative that self-assembles to form ribozyme oligomers. In a comparative activity assay using PEG as a molecular crowder, PEG rescued mutations in the parent bimolecular ribozyme more effectively than those in the oligomeric form.
在 RNA 世界中，自我复制 RNA 的富集对它们的生存、扩增和进化是有益的。RNA 的自我组装可能是它们自我富集的一种策略。我们研究了分子拥挤对双分子 I 类核酶及其自组装形成核酶寡聚物的衍生物的活性的影响。在使用 PEG 作为分子拥挤剂的比较活性测定中，PEG 对双亲本双分子核酶突变的拯救效果优于寡聚形式的突变。
