寡聚化的一个模块化核酶组装，这是由两个结构模块之间的可编程 RNA-RNA 界面控制的。

Oligomerization of a modular ribozyme assembly of which is controlled by a programmable RNA-RNA interface between two structural modules.

机构信息

Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, 3190 Gofuku, Toyama 930-8555, Japan.

Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi-ku, Fukuoka 819-0395, Japan.

出版信息

J Biosci Bioeng. 2019 Oct;128(4):410-415. doi: 10.1016/j.jbiosc.2019.04.003. Epub 2019 May 17.

DOI:10.1016/j.jbiosc.2019.04.003

PMID:31109874

Abstract

Bimolecular ribozymes derived by physical dissection of unimolecular ribozymes consisting of two structural modules are promising platforms for the design and construction of assembled RNA nanostructures. Unit RNAs to be assembled intermolecularly into one-dimensional (1D) oligomers are designed by reconnecting the two structural modules in a manner different from the parent ribozymes. This strategy was applied to the Tetrahymena group I ribozyme. We constructed 1D ribozyme oligomers the assembly of which was observed by atomic force microscopy (AFM) and also controlled rationally to design a heterooctamer by differentiating the interface between the two modules.

摘要

由两个结构模块组成的单分子核酶经物理切割得到的双分子核酶，为组装 RNA 纳米结构的设计和构建提供了有前途的平台。通过与亲本核酶不同的方式重新连接两个结构模块，设计出要通过分子间相互作用组装成一维（1D）寡聚物的单元 RNA。该策略应用于四膜虫 I 组核酶。我们构建了 1D 核酶寡聚物，通过原子力显微镜（AFM）观察到它们的组装，并且还通过区分两个模块之间的界面来进行理性控制，以设计异八聚体。