Oi Hiroki, Fujita Daisuke, Suzuki Yuki, Sugiyama Hiroshi, Endo Masayuki, Matsumura Shigeyoshi, Ikawa Yoshiya
Department of Chemistry, Graduate School of Science and Engineering, University of Toyama, Gofuku 3190, Toyama 930-8555, Japan.
Department of Chemistry, Graduate School of Science, Kyoto University, Kyoto 606-8502, Japan.
J Biochem. 2017 May 1;161(5):451-462. doi: 10.1093/jb/mvw093.
RNA is a biopolymer that is attractive for constructing nano-scale objects with complex structures. Three-dimensional (3D) structures of naturally occurring RNAs often have modular architectures. The 3D structure of a group I (GI) ribozyme from Tetrahymena has a typical modular architecture, which can be separated into two structural modules (ΔP5 and P5abc). The fully active ribozyme can be reconstructed by assembling the two separately prepared modules through highly specific and strong assembly between ΔP5 ribozyme and P5abc RNA. Such non-covalent assembly of the two modules allows the design of polygonal RNA nano-structures. Through rational redesign of the parent GI ribozyme, we constructed variant GI ribozymes as unit RNAs for polygonal-shaped (closed) oligomers with catalytic activity. Programmed trimerization and tetramerization of the unit RNAs afforded catalytically active nano-sized RNA triangles and squares, the structures of which were directly observed by atomic force microscopy (AFM).
RNA是一种生物聚合物，对于构建具有复杂结构的纳米级物体具有吸引力。天然存在的RNA的三维（3D）结构通常具有模块化架构。来自嗜热四膜虫的I组（GI）核酶的3D结构具有典型的模块化架构，可分为两个结构模块（ΔP5和P5abc）。通过在ΔP5核酶和P5abc RNA之间进行高度特异性和强组装，将两个单独制备的模块组装在一起，可以重建完全活性的核酶。两个模块的这种非共价组装允许设计多边形RNA纳米结构。通过对亲本GI核酶进行合理重新设计，我们构建了变体GI核酶作为具有催化活性的多边形（封闭）寡聚体的单元RNA。单元RNA的程序化三聚化和四聚化产生了具有催化活性的纳米级RNA三角形和正方形，其结构通过原子力显微镜（AFM）直接观察到。
