Deng Yi Zhen, Zhang Bin, Chang Changqing, Wang Yixu, Lu Shan, Sun Shuquan, Zhang Xiaomeng, Chen Baoshan, Jiang Zide
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/Integrative Microbiology Research Centre, South China Agricultural University, Guangzhou, China.
Department of Plant Pathology/Guangdong Province Key Laboratory of Microbial Signals and Disease Control, South China Agricultural University, Guangzhou, China.
Front Microbiol. 2018 Oct 26;9:2555. doi: 10.3389/fmicb.2018.02555. eCollection 2018.
In the phytopathogenic fungus , sexual mating between two compatible haploid cells and the subsequent formation of dikaryotic hyphae is essential for infection. This process was shown to be commonly regulated by a mitogen-activated protein kinase (MAPK) and a cAMP/PKA signaling pathway in the corn smut fungus but remains largely unknown in . In this study, we identified a conserved putative MAP kinase Kpp2 in and named it as SsKpp2. The Δ mutant displayed significant reduction in mating/filamentation, which could be partially restored by addition of cAMP or tryptophol, a quorum-sensing molecule identified in budding yeast. Transcriptional profiling showed that genes governing mating or tryptophol biosynthesis were significantly differentially regulated in the Δ mutant compared to the WT, under mating condition. Our results demonstrate that the MAP kinase SsKpp2 is required for mating/filamentation likely through regulating the conserved pheromone signal transduction pathway and tryptophol production.
在植物病原真菌中，两个相容的单倍体细胞之间的有性交配以及随后双核菌丝的形成对于感染至关重要。在玉米黑粉菌中，这一过程通常由丝裂原活化蛋白激酶（MAPK）和cAMP/PKA信号通路调控，但在[此处原文缺失真菌名称]中仍 largely unknown（此处原文表述有误，推测可能是“很大程度上未知”）。在本研究中，我们在[此处原文缺失真菌名称]中鉴定出一个保守的假定MAP激酶Kpp2，并将其命名为SsKpp2。Δ突变体在交配/菌丝形成方面显著减少，添加cAMP或色醇（一种在芽殖酵母中鉴定出的群体感应分子）可部分恢复。转录谱分析表明，在交配条件下，与野生型相比，Δ突变体中控制[此处原文缺失真菌名称]交配或色醇生物合成的基因存在显著差异调控。我们的结果表明，MAP激酶SsKpp2可能通过调节保守的信息素信号转导途径和色醇产生，参与[此处原文缺失真菌名称]的交配/菌丝形成。
