Department of Otorhinolaryngology and Laryngological Oncology, Medical University of Silesia, Zabrze, Poland.
Department of Cytobiology, Collegium Medicum, Jagiellonian University, Krakow, Poland.
Biomed Res Int. 2018 Oct 17;2018:2610637. doi: 10.1155/2018/2610637. eCollection 2018.
The aim of this study was to evaluate a novel composite material for tracheal reconstruction in an ovine model. A polymer containing various forms of carbon fibers (roving, woven, and nonwoven fabric) impregnated with polysulfone (PSU) was used to create cylindrical tracheal implants, 3 cm in length and 2.5 cm in diameter. Each implant, reinforced with five rings made of PSU-impregnated carbon-fiber roving, had three external layers made of carbon-fiber woven fabric and the inner layer formed of carbon-fiber nonwoven fabric. The inner surface of five implants was additionally coated with polyurethane (PU), to promote migration of respiratory epithelium. The implants were used to repair tracheal defects (involving four tracheal rings) in 10 sheep (9-12 months of age; 40-50 kg body weight). Macroscopic and microscopic characteristics of the implants and tracheal anastomoses were examined 4 and 24 weeks after implantation. At the end of the follow-up period, outer surfaces of the implants were covered with the tissue which to various degree resembled histological structure of normal tracheal wall. In turn, inner surfaces of the prostheses were covered only with vascularized connective tissue. Inner polyurethane coating did not improve the outcomes of tracheal reconstruction and promoted excessive granulation, which contributed to moderate to severe stenosis at the tracheal anastomoses. The hereby presented preliminary findings constitute a valuable source of data for future research on a tracheal implant being optimally adjusted for medical needs.
本研究旨在评估一种新型复合材料在绵羊模型中的气管重建效果。该复合材料由含有各种形式碳纤维（长丝、机织和无纺纤维）的聚合物和聚砜（PSU）组成，用于制造长度为 3 厘米、直径为 2.5 厘米的圆柱形气管植入物。每个植入物都用五圈 PSU 浸渍碳纤维长丝制成的环进行加固，外部有三层碳纤维机织织物，内层由碳纤维无纺纤维制成。五个植入物的内表面还涂有聚氨酯（PU），以促进呼吸上皮的迁移。将这些植入物用于修复 10 只绵羊（9-12 个月龄；40-50 公斤体重）的气管缺损（涉及四个气管环）。在植入后 4 周和 24 周检查植入物和气管吻合口的宏观和微观特征。在随访期末，植入物的外表面被组织覆盖，这些组织在不同程度上类似于正常气管壁的组织学结构。相反，假体的内表面仅被血管化的结缔组织覆盖。内聚氨酯涂层并没有改善气管重建的效果，反而促进了过度的肉芽形成，导致气管吻合口出现中度至重度狭窄。本研究初步结果为未来针对最佳满足医疗需求的气管植入物的研究提供了有价值的数据来源。
