Li Mufan, Zhang Eryang, Lü Leifeng, Ban Wenrui, Dang Xiaoqian, Zhang Chen
The First Department of Orthopaedics, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710004, P.R.China.
The First Department of Orthopaedics, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an Shaanxi, 710004,
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2018 Nov 1;32(11):1421-1428. doi: 10.7507/1002-1892.201801046.
To investigate the possibility of mitochondria-dependent apoptosis as a mechanism of early steroid-induced avascular necrosis of femoral head (SANFH) in rats and vitamin E as a possible prevention strategy.
Seventy-two male Sprague Dawley rats were randomly divided into control group, model group, and intervention group, with 24 rats in each group. The rats in control group were not treated as normal control. The rats in model group and intervention group were established early SANFH models by lipopolysaccharide combined with methylprednisolone injection. At the same time, the rats in intervention group were injected with vitamin E (40 mg/kg) every day for 7 days. At 2, 4, and 8 weeks after the final injection, the bilateral femoral heads were harvested and observed by HE staining, TUNEL assay, immunohistochemical staining, and Western blot. The rate of empty lacunae, apoptotic index, and the expressions of Caspase-9, Caspase-3, and cytochrome-c (Cyt-c) proteins were calculated.
According to histological staining, there were significant differences in the rate of empty lacunae between intervention group and control group at 8 weeks ( <0.05) and between intervention group and model group at 4 and 8 weeks ( <0.05). The apoptotic index of intervention group was significantly lower than that of model group at each time point ( <0.05). And there was significant difference between the intervention group and the control group at 8 weeks ( <0.05). According to immunohistochemistry staining and Western blot, the expressions of Cyt-c, Caspase-9, and Caspase-3 all significantly decreased in intervention group than those in model group at each time point ( <0.05); and the differences were significant between intervention group and control group at 8 weeks ( <0.05).
Vitamin E can delay the progression of early SANFH by reducing mitochondrial dependent osteocyte apoptosis.
探讨线粒体依赖性凋亡作为大鼠早期类固醇诱导的股骨头缺血性坏死（SANFH）机制的可能性，以及维生素E作为一种可能的预防策略。
72只雄性Sprague Dawley大鼠随机分为对照组、模型组和干预组，每组24只。对照组大鼠不做处理作为正常对照。模型组和干预组大鼠通过脂多糖联合甲基强的松龙注射建立早期SANFH模型。同时，干预组大鼠每天注射维生素E（40mg/kg），共7天。在最后一次注射后2、4和8周，采集双侧股骨头，进行苏木精-伊红（HE）染色、TUNEL检测、免疫组织化学染色和蛋白质免疫印迹法检测。计算空骨陷窝率、凋亡指数以及半胱天冬酶-9（Caspase-9）、半胱天冬酶-3（Caspase-3）和细胞色素c（Cyt-c）蛋白的表达。
组织学染色显示，干预组与对照组在8周时空骨陷窝率有显著差异（<0.05），干预组与模型组在4周和8周时空骨陷窝率有显著差异（<0.05）。干预组在各时间点的凋亡指数均显著低于模型组（<0.05），且干预组与对照组在8周时有显著差异（<0.05）。免疫组织化学染色和蛋白质免疫印迹法检测结果显示，干预组在各时间点Cyt-c、Caspase-9和Caspase-3的表达均显著低于模型组（<0.05）；干预组与对照组在8周时有显著差异（<0.05）。
维生素E可通过减少线粒体依赖性骨细胞凋亡来延缓早期SANFH的进展。
