EDTA 预处理提高自酸蚀黏结剂长期微拉伸粘结强度对硬化牙本质的粘结效果。

EDTA Conditioning Increases the Long-term Microtensile Bond Strength to Sclerotic Dentin Mediated by Self-etch Adhesives.

出版信息

J Adhes Dent. 2018;20(5):397-403. doi: 10.3290/j.jad.a41358.

DOI:10.3290/j.jad.a41358

Abstract

PURPOSE

To evaluate the immediate and 12-month microtensile bond strength (μTBS) of two self-etch adhesives on sclerotic dentin with or without previous EDTA conditioning. The conditioning pattern and the relative area of open dentinal tubules were also evaluated.

MATERIALS AND METHODS

Twenty-eight bovine incisors with naturally exposed sclerotic dentin were used. For μTBS testing, 20 teeth were divided into 4 groups (n = 5) according to the combination of the main factors: 1. adhesive (Clearfil SE Bond [CSE, Kuraray] and Adper SE Plus [ADSE, 3M Oral Care]); 2. surface treatment (previous conditioning with EDTA and previous conditioning with distilled water [DW]). The sclerotic surfaces were conditioned with either 17% EDTA or DW for 2 min prior to adhesive application. Composite buildups were constructed. Specimens were sectioned to obtain composite-dentin sticks (0.8 mm2) to be tested immediately or after 12 months (distilled water at 37°C) in tensile mode (0.5 mm/min). The conditioning pattern and the relative area of open dentinal tubules (OT) were evaluated in the remaining eight teeth. After adhesive application, the surfaces were rinsed off with acetone and ethanol. Then the surface was evaluated by SEM with image software. Data from μTBS and OT were submitted to three-way repeated measures ANOVA and data from OT were submitted to one-way repeated measures ANOVA. After, for both tests, Tukey's post-hoc test was applied (α = 0.05).

RESULTS

For both adhesives, EDTA resulted in the highest mean immediate μTBS (p = 0.002), which remained stable after 12 months of water storage (p < 0.38). CSE showed higher μTBS when compared to ADSE (p = 0.001). Degradation of the mean μTBS was observed for both adhesives only in the DW groups. Adhesive application plus with EDTA conditioning increased the OT area in comparison with only adhesive application (p < 0.001).

CONCLUSION

EDTA conditioning increased the immediate microtensile bond strength in sclerotic dentin substrate and prevented degradation when associated with a self-etch adhesive.

摘要

目的

评估两种自酸蚀粘结剂在有或没有 EDTA 预处理的情况下对硬化牙本质的即刻和 12 个月微拉伸粘结强度（μTBS）。还评估了预处理模式和开放牙本质小管的相对面积。

材料和方法

使用 28 颗具有天然暴露硬化牙本质的牛切牙。为了进行 μTBS 测试，20 颗牙齿根据主要因素的组合分为 4 组（n = 5）：1. 粘结剂（Clearfil SE Bond [CSE，Kuraray] 和 Adper SE Plus [ADSE，3M Oral Care]）；2. 表面处理（EDTA 预处理和去离子水预处理）。在应用粘结剂之前，将硬化表面用 17% EDTA 或去离子水预处理 2 分钟。构建复合修复体。将标本切割成 0.8mm2 的复合-牙本质棒，在拉伸模式下（0.5mm/min）立即或 12 个月后（37°C 去离子水）进行测试。在剩余的 8 颗牙齿中评估预处理模式和开放牙本质小管（OT）的相对面积。在应用粘结剂后，用丙酮和乙醇冲洗掉表面。然后用 SEM 和图像软件评估表面。μTBS 和 OT 的数据进行三向重复测量方差分析，OT 的数据进行单向重复测量方差分析。之后，对于这两种测试，都应用 Tukey 事后检验（α = 0.05）。

结果

对于两种粘结剂，EDTA 预处理均可获得最高的即刻 μTBS（p = 0.002），在 12 个月的水储存后仍保持稳定（p < 0.38）。与 ADSE 相比，CSE 表现出更高的 μTBS（p = 0.001）。只有在 DW 组中，两种粘结剂的平均 μTBS 都出现了降解。与仅应用粘结剂相比，应用粘结剂加 EDTA 预处理增加了 OT 面积（p < 0.001）。