El-Deeb Heba A, Daifalla Lamia E, Badran Omar I, Mobarak Enas H
J Adhes Dent. 2016;18(4):303-9. doi: 10.3290/j.jad.a36153.
To determine the influence of aging in artificial saliva compared to distilled water on the dentin microtensile bond strength (μTBS) of different adhesive systems.
Occlusal enamel and superficial dentin of 42 teeth were removed and roots were sectioned to expose the pulp chamber for connecting the tooth segments to an intrapulpal pressure assembly. According to the tested adhesives, tooth segments were allocated to three groups (n = 14): an etch-and-rinse adhesive (Adper Scotchbond Multi-Purpose, SBMP), a two-step self-etching adhesive (Clearfil SE Bond, CSE), and a singlestep self-etching adhesive (Clearfil S3 Bond, S3). Each adhesive system was applied to the dentin surface according to its manufacturer's instructions, while intrapulpal pressure was simulated. Resin composite (3M ESPE) was built up in two increments of 2 mm each. Each bonded specimen was sectioned to obtain eight sticks (0.8 ± 0.01 mm2). Sticks of each group were divided equally (n = 56) according to the storage solution, either distilled water or artificial saliva. For each storage solution, half of the sticks of each subgroup (n = 28) was stored for 24 h at 37°C and the other half was thermocycled for 10,000 cycles between 5°C and 55°C. Sticks were then subjected to μTBS testing. Data were statistically analyzed using multifactor ANOVA with repeated measures and Bonferroni's post-hoc test (p < 0.05). Student's t-test was used for pairwise comparison. Failure modes were determined for all tested sticks using scanning electron microscopy.
The decrease in bond strength of the three adhesives was significantly higher in distilled water than in artificial saliva. The predominant failure modes were adhesive and mixed.
The decrease in bond strength was more pronounced for specimens stored in distilled water than in artificial saliva.
确定与蒸馏水相比，人工唾液对不同粘结系统牙本质微拉伸粘结强度（μTBS）的老化影响。
去除42颗牙齿的咬合面釉质和表层牙本质，将牙根进行切片以暴露牙髓腔，从而将牙段连接至牙髓内压力组件。根据测试的粘结剂，将牙段分为三组（n = 14）：一种酸蚀冲洗粘结剂（Adper Scotchbond多功能粘结剂，SBMP）、一种两步自酸蚀粘结剂（Clearfil SE粘结剂，CSE）和一种一步自酸蚀粘结剂（Clearfil S3粘结剂，S3）。每种粘结系统均按照制造商的说明应用于牙本质表面，同时模拟牙髓内压力。用树脂复合材料（3M ESPE）分两次每次2 mm堆积。将每个粘结标本切片以获得八根小棒（0.8±0.01 mm2）。根据储存溶液（蒸馏水或人工唾液），将每组小棒平均分为两组（n = 56）。对于每种储存溶液，每个亚组的一半小棒（n = 28）在37°C下储存24小时，另一半在5°C至55°C之间进行10000次热循环。然后对小棒进行μTBS测试。数据采用重复测量的多因素方差分析和Bonferroni事后检验进行统计学分析（p < 0.05）。采用学生t检验进行两两比较。使用扫描电子显微镜确定所有测试小棒的失败模式。
三种粘结剂在蒸馏水中的粘结强度下降明显高于在人工唾液中的下降。主要的失败模式为粘结性和混合性。
与储存在人工唾液中的标本相比，储存在蒸馏水中的标本粘结强度下降更为明显。
