IEEE Trans Biomed Circuits Syst. 2018 Dec;12(6):1337-1344. doi: 10.1109/TBCAS.2018.2880922. Epub 2018 Nov 12.
This paper presents the development of a compact optoelectronic device suitable for on-chip detection of fluorescent molecules. In order to obtain a highly integrated device, a long-pass multi-dielectric filter has been integrated with thin-film amorphous silicon photosensors on a single glass substrate. Filter rejects the excitation light, allowing the reduction of the distance between the source and the fluorescent site and avoiding the use of external optical component. The compatibility of the technological processes determined the materials and the temporal sequence of the device fabrication. The developed device has been designed for the fluorescence detection of ruthenium complex based molecules and tested, as a proof of concept, for the detection of double-stranded DNA down to 0.5 ng. Results demonstrate the correct operation of the integrated system in both rejecting the excitation light and in detecting the fluorescent signal, demonstrating the suitability of this optoelectronic platform in practical biomedical applications.
本文提出了一种适用于荧光分子片上检测的紧凑型光电设备的研制。为了获得高度集成的设备，长通多介质滤光片已与薄膜非晶硅光电传感器集成在单个玻璃基板上。滤光片可以滤除激发光，从而可以缩短光源与荧光点之间的距离，避免使用外部光学元件。工艺过程的兼容性决定了器件制造的材料和时间顺序。所开发的设备是为基于钌配合物的荧光检测而设计的，并已通过实验验证，可检测低至 0.5ng 的双链 DNA。结果表明，集成系统在滤除激发光和检测荧光信号方面均能正常工作，证明了这种光电平台在实际生物医学应用中的适用性。
