Engineering Research Center of Optical Instrument and System, The Ministry of Education, Shanghai Key Laboratory of Modern Optical System, University of Shanghai for Science and Technology, Shanghai 200093, China.
Shanghai Environmental Biosafety Instruments and Equipment Engineering Technology Research Center, University of Shanghai for Science and Technology, Shanghai 200093, China.
Molecules. 2023 Feb 23;28(5):2104. doi: 10.3390/molecules28052104.
Undoubtedly, SARS-CoV-2 has caused an outbreak of pneumonia that evolved into a worldwide pandemic. The confusion of early symptoms of the SARS-CoV-2 infection with other respiratory virus infections made it very difficult to block its spread, leading to the expansion of the outbreak and an unreasonable demand for medical resource allocation. The traditional immunochromatographic test strip (ICTS) can detect one analyte with one sample. Herein, this study presents a novel strategy for the simultaneous rapid detection of FluB/SARS-CoV-2, including quantum dot fluorescent microspheres (QDFM) ICTS and a supporting device. The ICTS could be applied to realize simultaneous detection of FluB and SARS-CoV-2 with one test in a short time. A device supporting FluB/SARS-CoV-2 QDFM ICTS was designed and had the characteristics of being safe, portable, low-cost, relatively stable, and easy to use, ensuring the device could replace the immunofluorescence analyzer in cases where there is no need for quantification. This device does not need to be operated by professional and technical personnel and has commercial application potential.
毫无疑问，SARS-CoV-2 引起了肺炎爆发，并演变成了全球性大流行。SARS-CoV-2 感染的早期症状与其他呼吸道病毒感染混淆，使得阻止其传播变得非常困难，导致疫情扩大和医疗资源配置不合理。传统的免疫层析测试条（ICTS）可以检测一个样本中的一种分析物。在此，本研究提出了一种用于同时快速检测 FluB/SARS-CoV-2 的新策略，包括量子点荧光微球（QDFM）ICTS 和配套设备。该 ICTS 可应用于短时间内一次检测即可实现 FluB 和 SARS-CoV-2 的同时检测。设计了一种支持 FluB/SARS-CoV-2 QDFM ICTS 的设备，具有安全、便携、低成本、相对稳定和易于使用的特点，确保在无需定量的情况下，该设备可以替代免疫荧光分析仪。该设备不需要由专业技术人员操作，具有商业应用潜力。
