School of Aerospace Engineering, Sapienza University of Rome, via Salaria n. 851/881, 00138 Rome, Italy.
Department of Chemistry, Sapienza University of Rome, p.le Aldo Moro n.5, 00185 Rome, Italy.
Biosensors (Basel). 2017 Dec 5;7(4):58. doi: 10.3390/bios7040058.
A lab-on-chip system, integrating an all-glass microfluidics and on-chip optical detection, was developed and tested. The microfluidic network is etched in a glass substrate, which is then sealed with a glass cover by direct bonding. Thin film amorphous silicon photosensors have been fabricated on the sealed microfluidic substrate preventing the contamination of the micro-channels. The microfluidic network is then made accessible by opening inlets and outlets just prior to the use, ensuring the sterility of the device. The entire fabrication process relies on conventional photolithographic microfabrication techniques and is suitable for low-cost mass production of the device. The lab-on-chip system has been tested by implementing a chemiluminescent biochemical reaction. The inner channel walls of the microfluidic network are chemically functionalized with a layer of polymer brushes and horseradish peroxidase is immobilized into the coated channel. The results demonstrate the successful on-chip detection of hydrogen peroxide down to 18 μM by using luminol and 4-iodophenol as enhancer agent.
我们开发并测试了一种微流控芯片系统，它集成了全玻璃微流控和片上光学检测。微流控网络刻蚀在玻璃基底上，然后通过直接键合用玻璃盖密封。在密封的微流控基底上制造了薄膜非晶硅光电传感器，以防止微通道受到污染。在使用前仅打开入口和出口即可使微流控网络变得可用，从而确保设备的无菌性。整个制造过程依赖于传统的光刻微加工技术，非常适合设备的低成本批量生产。通过实施化学发光生化反应，对微流控芯片系统进行了测试。微流控网络的内通道壁用聚合物刷层进行化学功能化，将辣根过氧化物酶固定在涂覆的通道中。结果表明，通过使用发光氨和 4-碘苯酚作为增强剂，成功地在芯片上检测到低至 18μM 的过氧化氢。
