Fassi-Fehri M M, Johnson D W, Taoudi A, Berrada J
Département de Microbiologie, Institut Agronomique et Vétérinaire Hassan II, Rabat, Maroc.
Ann Rech Vet. 1988;19(1):59-64.
An epidemiological survey on E coli and rotavirus associated diarrheas in one to twenty five days old calves and lambs was made in three regions: Rabat-Kenitra, Marrakech and Agadir. Isolated E coli K99 stains have been studied of a biochemical, serotypical (O antigen) and antibiotypical point of view. The identification of rotavirus was made by ELISA test. Persistence of K99 antigen and heat stable toxin A was examined after a conservation of 5 weeks at - 18 degrees C. The frequency of E coli K99 or rotavirus associated diarrheas is respectively 26.9% and 29.7% in calf, 10% and 30% in lamb. This incidence considerably decreases from the 20th day in calf and from the 11th day in lamb. It must be observed that 34.8% of cases of diarrheas in calf and 55% in lamb cannot be ascribed to investigated agents. Only 12 out of 42 E coli K99 strains belong to serogroups O101, O8 and O9. Preservation of strains to - 18 degrees C comes with the loss of K99 antigen. These strains are not toxinogens. Among the strains having kept this antigen, 29% are toxinogens. Surveyings of antibiotics resistance was discussed.
对拉巴特-凯尼特拉、马拉喀什和阿加迪尔三个地区1至25日龄犊牛和羔羊中与大肠杆菌和轮状病毒相关腹泻进行了流行病学调查。从生化、血清型(O抗原)和抗微生物型角度对分离出的大肠杆菌K99菌株进行了研究。通过ELISA试验鉴定轮状病毒。在-18℃保存5周后,检测K99抗原和热稳定毒素A的持久性。犊牛中与大肠杆菌K99或轮状病毒相关腹泻的发生率分别为26.9%和29.7%,羔羊中分别为10%和30%。这种发病率在犊牛出生后第20天和羔羊出生后第11天显著下降。必须注意到,犊牛腹泻病例中有34.8%、羔羊腹泻病例中有55%不能归因于所调查的病原体。42株大肠杆菌K99菌株中只有12株属于O101、O8和O9血清群。将菌株保存在-18℃会导致K99抗原丧失。这些菌株不产生毒素。在保留该抗原的菌株中,29%产生毒素。讨论了抗生素耐药性调查情况。