Institute of Veterinary Sciences, University of Saad Dahlab- Blida 1, Street Soumaa, BP 270, Blida, 09000, Algeria.
Institute of Veterinary Sciences, University of Saad Dahlab- Blida 1, Street Soumaa, BP 270, Blida, 09000, Algeria.
Comp Immunol Microbiol Infect Dis. 2020 Dec;73:101567. doi: 10.1016/j.cimid.2020.101567. Epub 2020 Oct 22.
The etiology of neonatal diarrhea is multifactorial and remains one of the greatest health problems in sheep livestock farming. Faecal samples from 559 neonatal lambs aged less than 30 days from 30 sheepfolds located in the north-center region of Algeria were screened with pathogen-specific antigen ELISA for Cryptosporidium parvum, Escherichia coli K99, rotavirus, and coronavirus. Of the 559 lambs, 312 (58.81 %), 155 (27.72 %), 72 (12.88 %) and 20 (3.57 %) were positives for C. parvum, E. coli K99, rotavirus and coronavirus antigens, respectively. The prevalence of C. parvum was the highest (p < 0.0001). C. parvum, E. coli K99, rotavirus and coronavirus were observed in 23 (76.66 %), 17 (56.66 %), 9 (30 %) and 3 (10 %) sheepfolds, respectively. Compared to age, the prevalence of C. parvum was highest during the second and third week of age (p < 0.001). In contrast, other pathogens were found to be more frequent in lambs aged ≤7 days (p < 0.001). The number of lambs with diarrhea was 280 (50.09 %) of which 280 (100 %), 127 (45.35 %), 52 (18.57 %) and 10 (3.57 %) were found to be infected with C. parvum, E. coli K99, rotavirus and coronavirus, respectively (p < 0.0001). In various combinations, mixed infections were detected only with C. parvum. This is the first report of C. parvum, E. coli K99, rotavirus, and coronavirus in ≤30-days old neonatal lambs in Algeria. Special attention should be given to the first colostrum feeding, hygiene of the farm, prevention and control measures for a better prevention of neonatal diarrhea in lambs.
新生儿腹泻的病因是多因素的，仍然是绵羊养殖业中最大的健康问题之一。从阿尔及利亚中北部地区 30 个绵羊场的 559 只年龄小于 30 天的新生羔羊的粪便样本中，使用针对小隐孢子虫、大肠杆菌 K99、轮状病毒和冠状病毒的病原体特异性抗原 ELISA 进行了筛选。在 559 只羔羊中，小隐孢子虫、大肠杆菌 K99、轮状病毒和冠状病毒抗原的阳性率分别为 312 只（58.81%）、155 只（27.72%）、72 只（12.88%）和 20 只（3.57%）。小隐孢子虫的流行率最高（p < 0.0001）。在 23 个（76.66%）、17 个（56.66%）、9 个（30%）和 3 个（10%）绵羊场中观察到小隐孢子虫、大肠杆菌 K99、轮状病毒和冠状病毒。与年龄相比，小隐孢子虫在第 2 周和第 3 周的流行率最高（p < 0.001）。相比之下，其他病原体在≤7 天大的羔羊中更为常见（p < 0.001）。腹泻羔羊的数量为 280 只（50.09%），其中 280 只（100%）、127 只（45.35%）、52 只（18.57%）和 10 只（3.57%）分别感染了小隐孢子虫、大肠杆菌 K99、轮状病毒和冠状病毒（p < 0.0001）。在各种组合中，仅检测到小隐孢子虫的混合感染。这是在阿尔及利亚≤30 天大的新生羔羊中首次报告小隐孢子虫、大肠杆菌 K99、轮状病毒和冠状病毒。应特别注意首次初乳喂养、农场卫生、预防和控制措施，以更好地预防羔羊的新生儿腹泻。
