Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin, China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Front Cell Infect Microbiol. 2018 Oct 29;8:383. doi: 10.3389/fcimb.2018.00383. eCollection 2018.
Biofilms of are ubiquitously found on surfaces of many medical devices, which are the major cause of hospital-acquired infections. A large amount of work has been focused on bacterial attachment on surfaces. However, how bacterial cells evolve on surfaces after their attachment is the key to get better understanding and further control of biofilm formation. In this work, by employing both single-cell- and collective-motility of cells, we characterized the bacterial surface movement on physiochemically distinct surfaces. The measurement of cell surface motility showed consistent results that gold and especially platinum surfaces displayed a stronger capability in microcolony formation than polyvinyl chloride and polycarbonate surfaces. More interestingly, we found that overproduction of Psl led to a narrower variance in cell surface motility among tested surfaces, indicating an overshadow effect of Psl for bacteria by screening the influence of physicochemical properties of solid surfaces. Our results provide insights into how cells adapt their motion to physiochemically distinct surfaces, and thus would be beneficial for developing new anti-biofouling techniques in biomedical engineering.
生物膜在许多医疗器械的表面无处不在，是医院获得性感染的主要原因。大量的工作集中在细菌对表面的附着上。然而，细菌细胞在附着后的进化是更好地理解和进一步控制生物膜形成的关键。在这项工作中，我们通过单个细胞和细胞集体运动，对细菌在物理化学性质不同的表面上的表面运动进行了表征。细胞表面运动的测量结果一致表明，金，尤其是铂表面比聚氯乙烯和聚碳酸酯表面具有更强的微菌落形成能力。更有趣的是，我们发现 Psl 的过度表达导致在测试表面上细胞表面运动的方差变窄，这表明 Psl 通过筛选固体表面物理化学性质的影响对细菌产生了遮蔽效应。我们的结果提供了关于如何使 细胞适应物理化学性质不同的表面的运动的见解，这将有助于开发生物医学工程中的新型抗生物污染技术。
