评价基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF VITEK MS）和 VITEK 2 系统对腐生葡萄球菌的鉴定能力。
Evaluation of MALDI-TOF VITEKMS and VITEK 2 system for the identification of Staphylococcus saprophyticus.
机构信息
Department of Microbiology & Immunology, Botucatu Biosciences Institute, UNESP - Univ. Estadual Paulista, Botucatu, SP, Brazil.
Laboratory of Microbiology, University Hospital of the Botucatu School of Medicine (HC-FMB), UNESP - Univ. Estadual Paulista, Botucatu, SP, Brasil.
出版信息
Future Microbiol. 2018 Nov;13:1603-1609. doi: 10.2217/fmb-2018-0195. Epub 2018 Nov 13.
AIM
To compare two identification methods for coagulase-negative staphylococci (CoNS) isolated from patients with urinary tract infections, VITEK 2 and MALDI-TOF VITEKMS, with genotypic identification by internal transcribed spacer PCR (ITS-PCR).
RESULTS
A total of 217 CoNS isolates were studied. Agreement of the VITEK 2 system with ITS-PCR was 84.8%, with 98% sensitivity and 100% specificity. Thirty-one of the 33 strains incorrectly identified by VITEK 2 belonged to the species Staphylococcus saprophyticus. MALDI-TOF VITEKMS showed an excellent correlation with ITS-PCR since it correctly identified all CoNS isolates.
CONCLUSION
MALDI-TOF VITEKMS is more accurate than the automated VITEK 2 system in identifying CoNS isolated from urinary tract infections to species level, particularly urinary isolates of S. saprophyticus.
目的
比较两种凝固酶阴性葡萄球菌（CoNS）鉴定方法，即 VITEK 2 和 MALDI-TOF VITEKMS，与基因分型鉴定（ITS-PCR），用于鉴定尿路感染患者分离的 CoNS。
结果
共研究了 217 株 CoNS 分离株。VITEK 2 系统与 ITS-PCR 的一致性为 84.8%，具有 98%的敏感性和 100%的特异性。33 株被 VITEK 2 错误鉴定的菌株中有 31 株属于腐生葡萄球菌。MALDI-TOF VITEKMS 与 ITS-PCR 相关性极好，因为它正确鉴定了所有 CoNS 分离株。
结论
MALDI-TOF VITEKMS 比自动 VITEK 2 系统更准确地鉴定尿路感染患者分离的 CoNS 到种水平，特别是尿源腐生葡萄球菌。
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