URMITE, UM63, CNRS 7278, IRD 198, INSERM 1095, IHU-Méditerranée Infection, Aix-Marseille Université, 19-21 Boulevard Jean Moulin, 13385, Marseille Cedex 05, France.
Pôle des Maladies Infectieuses et Tropicales Clinique et Biologique, Fédération de Bactériologie-Hygiène-Virologie, University Hospital Centre Timone, Institut Hospitalo-Universitaire (IHU) Méditerranée Infection, Assistance Publique - Hôpitaux de Marseille, Marseille, France.
Eur J Clin Microbiol Infect Dis. 2017 Dec;36(12):2371-2377. doi: 10.1007/s10096-017-3069-6. Epub 2017 Aug 22.
Staphylococcus saprophyticus is one of the leading causes of urinary tract infections (UTI). In December 2014, our surveillance system identified an abnormal increase in S. saprophyticus causing UTIs in four university hospitals in Marseille, indicating a suspected community S. saprophyticus UTI outbreak. This was detected by our surveillance system BALYSES (Bacterial real-time Laboratory-based Surveillance System). S. saprophyticus/ Escherichia coli UTI ratio increased three-fold from 0.0084 in 2002 to 0.025 in December 2015 in Marseille with an abnormal peak in December 2014, and with an annual estimated ratio trend of 5.10 (p-value < 10). Matrix-Assisted Laser Desorption Ionisation-Time of Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis of strains was used to analyse strains cluster expansion, comparing strains from Marseille to those from Nice during the same period. MALDI-TOF MS spectral analysis revealed a geographical restricted clonal expansion of the strains clusters in Marseille as compared to Nice. Our finding suggests (i) a geographically restricted expansion of a specific S. saprophyticus strain clusters circulating in Marseille, and (ii) MALDI-TOF MS can be used as a cost-effective tool to investigate an outbreak.
腐生葡萄球菌是尿路感染（UTI）的主要病因之一。2014 年 12 月，我们的监测系统发现，马赛的 4 所大学医院中，腐生葡萄球菌引起的 UTIs 异常增加，表明疑似发生社区腐生葡萄球菌 UTI 暴发。这是通过我们的监测系统 BALYSES（基于实验室的实时细菌监测系统）检测到的。2002 年，腐生葡萄球菌/大肠埃希菌 UTI 比值为 0.0084，2015 年 12 月增至 0.025，2014 年 12 月出现异常峰值，年估计比值趋势为 5.10（p 值<10）。对菌株进行基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）光谱分析，以分析菌株聚类的扩展情况，并将同期马赛和尼斯的菌株进行比较。MALDI-TOF MS 光谱分析显示，与尼斯相比，马赛的菌株聚类呈地理上受限的扩展。我们的研究结果表明：（i）在马赛流行的特定腐生葡萄球菌菌株群呈地理上受限的扩展；（ii）MALDI-TOF MS 可作为一种具有成本效益的工具，用于调查暴发事件。
