School of Pharmacy, Collaborative Innovation Center of Sichuan for Elderly Care and Health, Chengdu Medical College, Chengdu 610500, China.
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Molecules. 2018 Nov 9;23(11):2921. doi: 10.3390/molecules23112921.
In the present study, 11 non-anthocyanin polyphenols, gallic acid, protocatechuate, vanillic acid, syringic acid, ferulic acid, quercetin, catechin, epicatechin, epigallocatechin gallate, gallocatechin gallate and epicatechin gallate-were firstly screened and identified from blueberries using an ultra performance liquid chromatography⁻time of flight mass spectrography (UPLC-TOF/MS) method. Then, a sample preparation method was developed based on vortex-assisted dispersive liquid-liquid microextraction. The microextraction conditions, including the amount of ethyl acetate, the amount of acetonitrile and the solution pH, were optimized through the multi-objective response surface methodology and desirability function approach. Finally, an ultra performance liquid chromatography⁻triple quadrupole mass spectrography (UPLC-QqQ/MS) method was developed to determine the 11 non-anthocyanin polyphenols in 25 commercial blueberry samples from Sichuan province and Chongqing city. The results show that this new method with high accuracy, good precision and simple operation characteristics, can be used to determine non-anthocyanin polyphenols in blueberries and is expected to be used in the analysis of other fruits and vegetables.
在本研究中，首次使用超高效液相色谱⁻飞行时间质谱联用（UPLC-TOF/MS）法从蓝莓中筛选并鉴定了 11 种非花青素多酚，分别为没食子酸、原儿茶酸、香草酸、丁香酸、阿魏酸、槲皮素、儿茶素、表儿茶素、表没食子儿茶素没食子酸酯、没食子儿茶素没食子酸酯和表儿茶素没食子酸酯。然后，基于涡旋辅助分散液液微萃取法开发了一种样品制备方法。通过多目标响应面法和理想性函数方法对微萃取条件（包括乙酸乙酯的用量、乙腈的用量和溶液 pH 值）进行了优化。最后，建立了一种超高效液相色谱⁻三重四极杆质谱联用（UPLC-QqQ/MS）法，用于测定来自四川省和重庆市的 25 个商业蓝莓样品中的 11 种非花青素多酚。结果表明，该方法具有准确度高、精密度好和操作简单的特点，可用于测定蓝莓中的非花青素多酚，有望用于其他水果和蔬菜的分析。
