Li Jie, Yuan Chunhua, Pan Li, Benatrehina P Annécie, Chai Heebyung, Keller William J, Naman C Benjamin, Kinghorn A Douglas
Division of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, The Ohio State University , 500 West 12th Avenue, Columbus, Ohio 43210, United States.
Nuclear Magnetic Resonance Facility, Campus Chemical Instrument Center, The Ohio State University , Columbus, Ohio 43210, United States.
J Agric Food Chem. 2017 Oct 4;65(39):8634-8642. doi: 10.1021/acs.jafc.7b03261. Epub 2017 Sep 26.
Bioassay-guided phytochemical investigation of a commercially available maqui berry (Aristotelia chilensis) extract used in botanical dietary supplement products led to the isolation of 16 compounds, including one phenolic molecule, 1, discovered for the first time from a natural source, along with several known compounds, 2-16, including three substances not reported previously in A. chilensis, 2, 14, and 15. Each isolate was characterized by detailed analysis of NMR spectroscopic and HRESIMS data and tested for their in vitro hydroxyl radical scavenging and quinone-reductase inducing biological activities. A sensitive and accurate LC-DAD-MS method for the quantitative determination of the occurrence of six bioactive compounds, 6, 7, 10-12, and 14, was developed and validated using maqui berry isolates purified in the course of this study as authentic standards. The method presented can be utilized for dereplication efforts in future natural product research projects or to evaluate chemical markers for quality assurance and batch-to-batch standardization of this botanical dietary supplement component.
对用于植物性膳食补充剂产品的市售智利酒果（Aristotelia chilensis）提取物进行生物测定指导的植物化学研究，导致分离出16种化合物，包括一种酚类分子（1，首次从天然来源发现）以及几种已知化合物（2 - 16），其中包括三种先前未在智利酒果中报道的物质（2、14和15）。通过对核磁共振光谱和高分辨电喷雾电离质谱数据的详细分析对每种分离物进行表征，并测试它们的体外羟基自由基清除和醌还原酶诱导生物活性。使用本研究过程中纯化的智利酒果分离物作为 authentic标准，开发并验证了一种灵敏且准确的液相色谱 - 二极管阵列 - 质谱方法，用于定量测定六种生物活性化合物（6、7、10 - 12和14）的含量。所提出的方法可用于未来天然产物研究项目中的重复排除工作，或评估该植物性膳食补充剂成分质量保证和批次间标准化的化学标志物。
