通过蛋白质免疫印迹法对杜克雷嗜血杆菌进行抗原分析。

Abeck D, Johnson A P, Taylor-Robinson D

Division of Sexually Transmitted Diseases, MRC Clinical Research Centre, Harrow, Middlesex.

Epidemiol Infect. 1988 Aug;101(1):151-7. doi: 10.1017/s0950268800029319.

Twenty-one strains of Haemophilus ducreyi were analysed by Western blotting using two antisera produced in mice. Common antigens of molecular weights 58, 46, 41, 28, 22 and 16 kDa were detected in all the strains. The antigens were protein in nature, since they could not be detected in whole-cell lysates which had been treated with proteinase K. The H. ducreyi strains showed antigenic cross-reactivity with strains of H. influenzae and H. parainfluenzae, but showed minimal or no cross-reactivity with seven other species of bacteria.

使用在小鼠体内产生的两种抗血清，通过蛋白质免疫印迹法对21株杜克雷嗜血杆菌进行了分析。在所有菌株中均检测到分子量为58、46、41、28、22和16 kDa的共同抗原。这些抗原本质上是蛋白质，因为在用蛋白酶K处理过的全细胞裂解物中无法检测到它们。杜克雷嗜血杆菌菌株与流感嗜血杆菌和副流感嗜血杆菌菌株表现出抗原交叉反应，但与其他七种细菌的交叉反应极小或无交叉反应。