通过间接免疫荧光试验对杜克雷嗜血杆菌进行分型。
Typing Haemophilus ducreyi by indirect immunofluorescence assay.
作者信息
Slootmans L, Vanden Berghe D A, Piot P
出版信息
Genitourin Med. 1985 Apr;61(2):123-6. doi: 10.1136/sti.61.2.123.
Abstract
Rabbits were inoculated with untreated whole cells of 16 strains of Haemophilus ducreyi. Homologous titres in an indirect immunofluorescence test ranged from 1/320 to 1/1280. No cross reactions, or very few, were observed between antisera to these strains and 22 other (possible taxonomically related) species, strains, and genera. A large diversity in types of antigen was observed among the strains of H ducreyi. Antiserum from two antigens (35000 and 3138), however, reacted with all 16 H ducreyi antigens tested. At least nine of the strains of H ducreyi examined could be identified as determining the type of antigen.
摘要
用16株杜克雷嗜血杆菌的未经处理的全细胞对兔子进行接种。间接免疫荧光试验中的同源滴度范围为1/320至1/1280。在这些菌株的抗血清与其他22个（可能在分类学上相关的）物种、菌株和属之间未观察到交叉反应，或者仅有极少的交叉反应。在杜克雷嗜血杆菌菌株中观察到抗原类型的巨大差异。然而，来自两种抗原（35000和3138）的抗血清与所有测试的16种杜克雷嗜血杆菌抗原发生反应。所检测的杜克雷嗜血杆菌菌株中至少有九种可被鉴定为决定抗原类型。
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1
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Ann Intern Med. 1981 Sep;95(3):315-8. doi: 10.7326/0003-4819-95-3-315.
2
Isolation and identification of Haemophilus ducreyi in a clinical study.一项临床研究中杜克雷嗜血杆菌的分离与鉴定
J Clin Microbiol. 1980 Aug;12(2):170-4. doi: 10.1128/jcm.12.2.170-174.1980.
3
Isolation and cultivation of Haemophilus ducreyi.杜克雷嗜血杆菌的分离与培养。
J Clin Microbiol. 1982 Apr;15(4):625-9. doi: 10.1128/jcm.15.4.625-629.1982.
4
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Sex Transm Dis. 1984 Jan-Mar;11(1):6-9. doi: 10.1097/00007435-198401000-00002.
5
Characterization of cell proteins of Haemophilus ducreyi by polyacrylamide gel electrophoresis.通过聚丙烯酰胺凝胶电泳对杜克雷嗜血杆菌细胞蛋白进行表征
J Infect Dis. 1983 Oct;148(4):710-4. doi: 10.1093/infdis/148.4.710.
6
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7
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