通过间接免疫荧光试验对杜克雷嗜血杆菌进行分型。
Typing Haemophilus ducreyi by indirect immunofluorescence assay.
作者信息
Slootmans L, Vanden Berghe D A, Piot P
出版信息
Genitourin Med. 1985 Apr;61(2):123-6. doi: 10.1136/sti.61.2.123.
Abstract
Rabbits were inoculated with untreated whole cells of 16 strains of Haemophilus ducreyi. Homologous titres in an indirect immunofluorescence test ranged from 1/320 to 1/1280. No cross reactions, or very few, were observed between antisera to these strains and 22 other (possible taxonomically related) species, strains, and genera. A large diversity in types of antigen was observed among the strains of H ducreyi. Antiserum from two antigens (35000 and 3138), however, reacted with all 16 H ducreyi antigens tested. At least nine of the strains of H ducreyi examined could be identified as determining the type of antigen.
摘要
用16株杜克雷嗜血杆菌的未经处理的全细胞对兔子进行接种。间接免疫荧光试验中的同源滴度范围为1/320至1/1280。在这些菌株的抗血清与其他22个（可能在分类学上相关的）物种、菌株和属之间未观察到交叉反应，或者仅有极少的交叉反应。在杜克雷嗜血杆菌菌株中观察到抗原类型的巨大差异。然而，来自两种抗原（35000和3138）的抗血清与所有测试的16种杜克雷嗜血杆菌抗原发生反应。所检测的杜克雷嗜血杆菌菌株中至少有九种可被鉴定为决定抗原类型。
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