Sarri Vitor Coca, Ferrari Beatriz Maria, Magalhães Larissa Fernandes, Rodrigues Paula Almeida, Rezende Almir Coelho, Brunherotti Marisa Afonso Andrade
Department of Medicine, Universidade de Franca, Franca, SP, Brazil.
Department of Veterinary Medicine, Universidade de Franca, Franca, SP, Brazil.
Rev Bras Ginecol Obstet. 2019 Jan;41(1):24-30. doi: 10.1055/s-0038-1675610. Epub 2018 Nov 14.
The aim of this study is to evaluate whether exposure to different environmental lighting conditions affects the reproductive parameters of pregnant mice and the development of their offspring.
Fifteen pregnant albino mice were divided into three groups: light/dark, light, and dark. The animals were euthanized on day 18 of pregnancy following the Brazilian Good Practice Guide for Euthanasia of Animals. Maternal and fetal specimens were measured and collected for histological evaluation. Analysis of variance (ANOVA) test was used for comparison of the groups considering ≤ 0.05 to be statistically significant.
There was no significant difference in the maternal variables between the three groups. Regarding fetal variables, significant differences were observed in the anthropometric measures between the groups exposed to different environmental lighting conditions, with the highest mean values in the light group. The histological evaluation showed the same structural pattern of the placenta in all groups, which was within the normal range. However, evaluation of the uterus revealed a discrete to moderate number of endometrial glands in the light/dark and light groups, which were poorly developed in most animals. In the fetuses, pulmonary analysis revealed morphological features consistent with the transition from the canalicular to the saccular phase in all groups.
Exposure to different environmental lighting conditions had no influence on the reproductive parameters of female mice, while the offspring of mothers exposed to light for 24 hours exhibited better morphometric features.
本研究旨在评估暴露于不同环境光照条件下是否会影响怀孕小鼠的生殖参数及其后代的发育。
将15只怀孕的白化小鼠分为三组：光照/黑暗组、光照组和黑暗组。按照巴西动物安乐死良好操作指南，在怀孕第18天对动物实施安乐死。测量并收集母体和胎儿标本进行组织学评估。采用方差分析（ANOVA）检验对各组进行比较，将P≤0.05视为具有统计学意义。
三组之间的母体变量无显著差异。关于胎儿变量，在暴露于不同环境光照条件的组之间，人体测量指标存在显著差异，光照组的平均值最高。组织学评估显示所有组的胎盘结构模式相同，均在正常范围内。然而，对子宫的评估显示，光照/黑暗组和光照组的子宫内膜腺体数量为少量至中等数量，大多数动物的腺体发育不良。在胎儿中，肺部分析显示所有组的形态学特征均与从细支气管期向囊状期的转变一致。
暴露于不同环境光照条件对雌性小鼠的生殖参数没有影响，而暴露于24小时光照的母体所生后代表现出更好的形态测量特征。
