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通过匀浆增强拭子中微生物的回收率:一种新型标准化高通量方法。

Augmented recovery of microorganisms from swabs by homogenization: a novel standardizable high-throughput approach.

作者信息

Warnke Philipp, Redanz Sylvio, Zaatreh Sarah, Podbielski Andreas

机构信息

Institute of Medical Microbiology, Virology, and Hygiene, Rostock University Hospital, Rostock, Germany.

Institute of Medical Microbiology, Virology, and Hygiene, Rostock University Hospital, Rostock, Germany.

出版信息

Diagn Microbiol Infect Dis. 2016 Jan;84(1):16-18. doi: 10.1016/j.diagmicrobio.2015.10.004. Epub 2015 Oct 9.

DOI:10.1016/j.diagmicrobio.2015.10.004
PMID:26514077
Abstract

A new approach introducing a quantitative and standardizable step into sample processing was evaluated by homogenizing in vitro inoculated swab tips with Precellys 24 high-throughput homogenizer. Recovery of microorganisms from homogenized swab tips was significantly higher as compared to conventional processing methods. Thus, swab homogenization is a promising approach introducing a new quality in microbial analysis.

摘要

通过使用Precellys 24高通量匀浆器对体外接种的拭子尖端进行匀浆,评估了一种在样品处理中引入定量和标准化步骤的新方法。与传统处理方法相比,从匀浆后的拭子尖端回收的微生物数量显著更高。因此,拭子匀浆是一种在微生物分析中引入新质量的有前景的方法。

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