R. C. van Gaal, Dr. P.-P. K. H. Fransen, Prof. P. Y. W. Dankers, Department of Biomedical Engineering, Laboratory of Chemical Biology and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, 5600 MB, Eindhoven, The Netherlands.
Dr. M. Fedecostante, Prof. R. Masereeuw, Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, Universiteitsweg, 99, 3584 CG, Utrecht, The Netherlands.
Macromol Biosci. 2019 Feb;19(2):e1800300. doi: 10.1002/mabi.201800300. Epub 2018 Nov 14.
Induction of a functional, tight monolayer of renal epithelial cells on a synthetic membrane to be applied in a bioartificial kidney device requires for bio-activation of the membrane. The current golden standard in bio-activation is the combination of a random polymeric catechol (L-DOPA) coating and collagen type IV (Col IV). Here the possibility of replacing this with defined monomeric catechol functionalization on a biomaterial surface using supramolecular ureido-pyrimidinone (UPy)-moieties is investigated. Monomeric catechols modified with a UPy-unit are successfully incorporated and presented in supramolecular UPy-polymer films and membranes. Unfortunately, these UPy-catechols are unable to improve epithelial cell monolayer formation over time, solely or in combination with Col IV. L-DOPA combined with Col IV is able to induce a tight monolayer capable of transport on electrospun supramolecular UPy-membranes. This study shows that a random polymeric catechol coating cannot be simply mimicked by defined monomeric catechols as supramolecular additives. There is still a long way to go in order to synthetically mimic simple natural structures.
诱导功能性、紧密的肾上皮细胞单层在合成膜上，以应用于生物人工肾设备，需要对膜进行生物活化。目前生物活化的金标准是将随机聚合儿茶酚（L-DOPA）涂层与 IV 型胶原（Col IV）结合。在这里，使用超分子尿嘧啶嘧啶酮（UPy）部分在生物材料表面取代这种方法的可能性，用定义的单体儿茶酚功能化进行了研究。成功地将带有 UPy 单元的单体儿茶酚掺入并呈现在超分子 UPy 聚合物薄膜和膜中。不幸的是，这些 UPy-儿茶酚单独或与 Col IV 结合，都无法随着时间的推移改善上皮细胞单层的形成。L-DOPA 与 Col IV 结合能够诱导能够在静电纺丝超分子 UPy 膜上进行运输的紧密单层。这项研究表明，作为超分子添加剂，定义明确的单体儿茶酚不能简单地模拟随机聚合儿茶酚涂层。为了合成模拟简单的天然结构，还有很长的路要走。
