Viot M, Cambon P, Blondel P
Laboratoire de Biologie, Centre Antoine-Lacassagne, Nice.
Pathol Biol (Paris). 1988 May;36(5):398-402.
Antimicrobial susceptibility testing of Streptococci in automated units (Autobac) is not always satisfactory: aggregates formed spontaneously by Streptococci in the medium used can reduce the reliability of tests by interfering with correct reading, and, in many cases, bacterial growth is very slow, necessitating long periods for analysis (up to 6 hours). Owing to the fact that use of "bead type" cassettes for antifungal susceptibility testing in the Autobac improved results, and in particular prevented the formation of aggregates, we decided to adopt the same system for tests of streptococci. Each compartment in these cassettes contains glass microbeads which prevent aggregate formation thanks to an agitation mechanism. Rapid sedimentation of these beads allows normal reading with the Autobac. A comparative study of 66 strains of streptococci with various antibiotics was performed. Three techniques were used: classical diffusion technique, susceptibility testing with the standard cassette in the Autobac, and test with the bead type cassette in the Autobac. Parameters evaluated included the effective duration of each test, and comparison of the results (Autobac with and without beads, diffusion method). Results are detailed and discussed.
在自动仪器（Autobac）中对链球菌进行药敏试验并不总是令人满意：链球菌在所用培养基中自发形成的聚集体会干扰正确读数，从而降低试验的可靠性，而且在许多情况下，细菌生长非常缓慢，需要很长时间进行分析（长达6小时）。由于在Autobac中使用“珠型”卡盒进行抗真菌药敏试验能改善结果，特别是能防止聚集体形成，我们决定对链球菌试验采用相同的系统。这些卡盒中的每个隔室都装有玻璃微珠，借助搅拌机制可防止聚集体形成。这些微珠的快速沉降使得能使用Autobac进行正常读数。对66株链球菌进行了不同抗生素的比较研究。采用了三种技术：经典扩散技术、在Autobac中使用标准卡盒进行药敏试验以及在Autobac中使用珠型卡盒进行试验。评估的参数包括每次试验的有效时长以及结果比较（有珠和无珠的Autobac、扩散法）。结果进行了详细阐述和讨论。
