Harf C, Perronnet F
Université Louis Pasteur, Faculté de Pharmacie, Strasbourg.
Pathol Biol (Paris). 1988 May;36(5):581-3.
Recent reports incriminating Acanthamoeba, a small free-living amoeba, wide-spread in environmental soils and waters, in acanthamoebic keratitis cases wearing soft contact lenses, drew attention to cleaning solutions for contact lenses. The purpose of this report is to discuss the amoebicidal action of a rinsing solution containing 0.001% thimerosal. A. castellanii cysts and trophozoites were incubated with the rinsing solution. Amoeba saline was used as control solution. After 1 h to 96 h of contact time, the survival was studied by growing of trophozoites and excystment of cysts on cultures. The results indicate that trophozoites were destroyed by the rinsing solution after 6 hours, whereas for cysts a negative culture was obtained after 72 h to 96 h. The amoebicidal and cysticidal action of this solution containing mercury corroborates our preceding experience on the effects of mercury derivates on amoebae. The use of amoebicidal solutions in contactology could inhibit and even destroy amoebae on contaminated lenses preventing severe keratitis.
最近有报告指出，棘阿米巴属这种小型自由生活阿米巴原虫广泛存在于环境土壤和水体中，在佩戴软性隐形眼镜的棘阿米巴角膜炎病例中涉及该病原体，这使得人们开始关注隐形眼镜清洁液。本报告的目的是探讨含有0.001%硫柳汞的冲洗液的杀阿米巴作用。将卡氏棘阿米巴包囊和滋养体与冲洗液一起孵育。阿米巴生理盐水用作对照溶液。在接触1小时至96小时后，通过在培养基上培养滋养体和包囊脱囊来研究其存活率。结果表明，滋养体在6小时后被冲洗液破坏，而对于包囊，在72小时至96小时后培养结果为阴性。这种含汞溶液的杀阿米巴和杀包囊作用证实了我们之前关于汞衍生物对阿米巴原虫作用的经验。在隐形眼镜学中使用杀阿米巴溶液可以抑制甚至破坏受污染镜片上的阿米巴原虫，预防严重的角膜炎。
