Labello Barbosa Rodrigo, Dias Viviane Liotti, Lorosa Elias Seixas, de Góes Costa Elenild, Pereira Karen Signori, Gilioli Rovilson, Guaraldo Ana Maria Aparecida, Passos Luiz Augusto Corrêa
Universidade Estadual de Campinas-Unicamp, Instituto de Biologia, Departamento de Biologia Animal - Parasitologia, Campinas, SP, Brazil.
Universidade Estadual de Campinas-Unicamp, Centro Multidisciplinar para Investigação Biológica na Área da Ciência de Animais de Laboratório-Cemib, Divisão de Pesquisa, Campinas, SP, Brazil.
Exp Parasitol. 2019 Feb;197:68-75. doi: 10.1016/j.exppara.2018.10.012. Epub 2018 Nov 12.
In recent decades some outbreaks of food-borne acute Chagas disease (ACD) in humans were identified by clinical and epidemiological characterization after association through the ingestion of açaí pulp probably contaminated with Trypanosoma cruzi. Whereas Belém and Abaetetuba stood out as important risk regions for disease transmission, the importance of Rhodnius pictipes, and Philander opossum for the biological cycle of T. cruzi, and data from agribusiness market of açaí, to study T. cruzi from vector and reservoir of the Brazilian Amazon region is critical for this context. Thus, the purpose of this study was to verify the infective capacity and the virulence of T. cruzi in açaí pulp from vector and reservoir at Pará State experimentally.
10T. cruzi I in in natura açaí pulp from Belém at Pará State, at room temperature, after forced sieving, by intraperitoneal, gavage or oral route of inoculation in B6.129S7Rag1/J Unib allowed food-borne ACD analysis using common light microscopy.
T. cruzi in in natura açaí pulp from R. pictipes (Val-De-Cans Forest, Belém, and Ajuaí River, Abaetetuba, Pará), and P. opossum (Combu Island, Belém, Pará) caused ACD and death between 17 and 52 days after experimental infections in murine immunodeficient hosts.
T. cruzi from different sources and locations at Pará State in in natura açaí pulp retained its infective capacity and virulence, and can cause new outbreaks of ACD by oral transmission. Additionally, quality basic education will facilitate efficient hygiene practices throughout the açaí productive chain can eradicate food-borne ACD in the coming decades.
近几十年来，通过临床和流行病学特征鉴定出了一些人类食源性急性恰加斯病（ACD）疫情，这些疫情是在摄入可能被克氏锥虫污染的阿萨伊果肉后关联起来的。虽然贝伦和阿贝泰图巴是疾病传播的重要风险地区，但红带锥蝽和南美负鼠对克氏锥虫生物循环的重要性，以及阿萨伊农产品市场的数据，对于研究巴西亚马逊地区媒介和宿主中的克氏锥虫在这种情况下至关重要。因此，本研究的目的是通过实验验证帕拉州媒介和宿主来源的阿萨伊果肉中克氏锥虫的感染能力和毒力。
将来自帕拉州贝伦的天然阿萨伊果肉中的10株克氏锥虫I，在室温下经过强制筛分后，通过腹腔注射、灌胃或口服途径接种到B6.129S7Rag1/J Unib小鼠体内，使用普通光学显微镜进行食源性ACD分析。
来自红带锥蝽（瓦尔迪坎斯森林、贝伦和阿茹艾河、阿贝泰图巴、帕拉）和南美负鼠（康布岛、贝伦、帕拉）的天然阿萨伊果肉中的克氏锥虫，在实验感染免疫缺陷小鼠宿主后17至52天内导致了ACD和死亡。
帕拉州不同来源和地点的天然阿萨伊果肉中的克氏锥虫保留了其感染能力和毒力，并可通过口服传播引发新的ACD疫情。此外，优质的基础教育将促进整个阿萨伊生产链的有效卫生习惯，在未来几十年内可以根除食源性ACD。
