Tahernia Mehdi, Mohammadifar Maedeh, Hassett Daniel J, Choi Seokheun
Annu Int Conf IEEE Eng Med Biol Soc. 2018 Jul;2018:1-4. doi: 10.1109/EMBC.2018.8513677.
For the first time, we report a low-cost, disposable fully-papertronic screening platform for rapid screening and identification of electroactive microorganisms. This novel papertronic device is capable of simultaneous characterizing the electrogenicity of 10' s of the newly discovered, genetically engineered, bacteria. This work explored an exciting range of possibilities with the goal of fusing microbial fuel cell technology with 'papertronics,' the emerging field of paper-based electronics. Spatially distinct 64 sensing units of the array were constructed by patterning hydrophilic anodic reservoirs in paper with hydrophobic wax boundaries and utilizing 3-D multi-laminate paper structures. Full integration of a high-performance microbial sensor on paper can be achieved by improving the microbial electron exchange with the electrodes in an engineered conductive paper reservoir and reducing cathodic overpotential by using a solid electron acceptor on paper. Furthermore, the intrinsic capillary force of the paper and the increased capacity from the engineered reservoir allowed for rapid adsorption of the bacterial sample and promote immediate microbial cell attachment to the electrode, leading to instant power generation with even a small amount of the liquid.
我们首次报道了一种低成本、一次性使用的全纸质电子筛查平台，用于快速筛查和鉴定电活性微生物。这种新型纸质电子设备能够同时表征数十种新发现的、经过基因工程改造的细菌的产电能力。这项工作探索了一系列令人兴奋的可能性，目标是将微生物燃料电池技术与“纸质电子学”（基于纸张的电子学新兴领域）融合。通过用疏水蜡边界在纸张上图案化亲水性阳极储液器并利用三维多层纸结构，构建了阵列中空间上不同的64个传感单元。通过在工程化的导电纸储液器中改善微生物与电极之间的电子交换，并在纸张上使用固体电子受体来降低阴极过电位，可以实现高性能微生物传感器在纸张上的完全集成。此外，纸张的固有毛细作用力以及工程化储液器增加的容量使得细菌样品能够快速吸附，并促进微生物细胞立即附着到电极上，即使使用少量液体也能立即发电。
