Tahernia Mehdi, Mohammadifar Maedeh, Hassett Daniel J, Choi Seokheun
Bioelectronics & Microsystems Laboratory, Department of Electrical & Computer Engineering, State University of New York-Binghamton, Binghamton, NY, 13902-6000, USA.
Department of Molecular Genetics, Biochemistry and Microbiology, University of Cincinnati College of Medicine, 231 Albert Sabin Way, Cincinnati, OH, 45267-0524, USA.
Biosens Bioelectron. 2020 Oct 1;165:112348. doi: 10.1016/j.bios.2020.112348. Epub 2020 Jun 10.
Electrogenic bacteria or exoelectrogens can transfer electrons to extracellular electron acceptors and thus have a wide range of applications to the ever-emerging fields of bioenergy, bioremediation, and biosensing. Standard state-of-the-art techniques for screening of electrogenic bacteria are inefficient, and often prevent rapid, high-throughput analyses. Herein, we created a simple, rapid, and straightforward papertronic 4- and 16-channel sensing platforms that is connected to a visual readout, allowing the naked eye to evaluate and quantify direct bacterial electrogenic capabilities. Our system integrated multiple 2-electrode sensing units into a signal amplifier circuit connected to light-emitting diode (LED) reporting units. The current generated from electrogenic bacteria in the sensing unit was amplified by the transistor and was transduced into LED illumination. The sensing units incorporated on the paper-based printed circuit boards (PCBs) absorbed bacteria-laden suspensions through capillary action, allowing for a rapid assessment (<2 min) of their electrogenic potential. Two well-known exoelectrogens, Shewanella oneidensis MR1 and Pseudomonas aeruginosa PA01, and many other mutants of the latter were selected to demonstrate the practicality of the proposed sensor. The effectiveness for on-site and portable measurements was validated by testing solid wastewater samples randomly obtained from the environment. Thus, the system described in this work highlights a novel form of a scalable, high-throughput sensing array for simple and rapid quantification of bacterial electrogenicity.
产电细菌或外生电菌能够将电子传递给细胞外电子受体，因此在生物能源、生物修复和生物传感等不断涌现的领域有着广泛应用。用于筛选产电细菌的标准先进技术效率低下，且常常阻碍快速、高通量分析。在此，我们创建了一种简单、快速且直接的纸质电子4通道和16通道传感平台，该平台连接到视觉读数装置，使肉眼能够评估和量化细菌的直接产电能力。我们的系统将多个两电极传感单元集成到一个与发光二极管（LED）报告单元相连的信号放大电路中。传感单元中产电细菌产生的电流由晶体管放大，并转换为LED照明。包含在纸质印刷电路板（PCB）上的传感单元通过毛细作用吸收含细菌的悬浮液，从而能够快速评估（<2分钟）它们的产电潜力。选择了两种著名的外生电菌，即希瓦氏菌属的MR-1和铜绿假单胞菌PA01，以及后者的许多其他突变体来证明所提出传感器的实用性。通过测试从环境中随机获取的固体废水样本，验证了该系统用于现场和便携式测量的有效性。因此，本文所述系统突出了一种新型的可扩展、高通量传感阵列形式，用于简单快速地量化细菌产电能力。
