Daish Christian, Blanchard Romane, Duchi Serena, Onofrillo Carmine, Augustine Cheryl, Fox Kate, Pivonka Peter, Pirogova Elena
Annu Int Conf IEEE Eng Med Biol Soc. 2018 Jul;2018:4166-4169. doi: 10.1109/EMBC.2018.8513239.
Pulsed electromagnetic field (PEMF) stimulation has been utilized in the medical field since the early 20th century. A number of therapeutic devices have been developed for the treatment of bone fractures and other medical applications. Most of these devices are backed by limited quantitative evidence. In this paper we present the development of a PEMF device for the purposes of determining, through in vitro experimentation, the exposure parameters required to give the most optimal fracture repair. Following electromagnetic field characterization, the device was shown to match well with computational field simulations. The exposure system has been validated through adipose-derived stem cell viability studies with an exposure frequency of 5 Hz and an intensity of 1.1 mT, for a duration of seven days at 30 minutes per day. Under the specific field characteristics chosen, the fatty-tissue derived stem cell proliferation was not hindered and in fact was stimulated $( 0. 025 < P < 0.01)$ by the PEMF exposure. With continued experimentation of numerous exposure conditions at the cellular scale, it will be possible to quantitatively determine the optimal exposure conditions required to produce the most rapid fresh fracture repair. Following this, there is significant potential for development of an optimized wearable device suitable for enhancing repair of all types of bone fractures.
自20世纪初以来，脉冲电磁场（PEMF）刺激已在医学领域得到应用。已经开发出许多治疗设备用于治疗骨折和其他医学应用。这些设备大多仅有有限的定量证据支持。在本文中，我们介绍了一种PEMF设备的研发情况，其目的是通过体外实验确定实现最优化骨折修复所需的暴露参数。经过电磁场特性表征，该设备被证明与计算场模拟结果匹配良好。通过脂肪来源干细胞活力研究对暴露系统进行了验证，暴露频率为5Hz，强度为1.1mT，每天暴露30分钟，持续7天。在所选的特定场特性下，脂肪组织来源的干细胞增殖未受阻碍，实际上PEMF暴露对其有刺激作用（0.025＜P＜0.01）。随着在细胞尺度上对众多暴露条件的持续实验，将有可能定量确定实现最快速新鲜骨折修复所需的最佳暴露条件。在此之后，开发一种适合增强各类骨折修复的优化可穿戴设备具有巨大潜力。
