Tian Kai, Chen Xiaowei, Luan Bingqun, Lin Mengshi, Mustapha Azlin, Gu Li-Qun
Annu Int Conf IEEE Eng Med Biol Soc. 2018 Jul;2018:4492-4495. doi: 10.1109/EMBC.2018.8513177.
Rapid and accurate detection of single-nucleotide polymorphism (SNP) in pathogenic mutants is crucial for broad fields from food safety monitoring to disease diagnostics and prognosis. Here, we developed a nanopore single-molecule sensor, coupled with the locked nucleic acid (LNA) technique, to accurately discriminate SNPs for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 pathogen serotype, and cancer-derived driver mutations EGFR L858R and KRAS G12D. This sensitive method, with a simplified, low cost, easy-to-operate LNA design, can be applied in food science and medical detection that need rapid and accurate determination of genetic variations.
快速准确地检测致病突变体中的单核苷酸多态性（SNP）对于从食品安全监测到疾病诊断和预后的广泛领域至关重要。在此，我们开发了一种与锁核酸（LNA）技术相结合的纳米孔单分子传感器，以准确区分SNP，用于检测产志贺毒素大肠杆菌（STEC）O157:H7病原体血清型以及癌症衍生的驱动突变EGFR L858R和KRAS G12D。这种灵敏的方法采用简化、低成本且易于操作的LNA设计，可应用于需要快速准确测定基因变异的食品科学和医学检测中。
