Knobloch J
Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Federal Republic of Germany.
Am J Trop Med Hyg. 1988 Aug;39(2):173-8. doi: 10.4269/ajtmh.1988.39.173.
Twenty-four antigens of Bartonella bacilliformis, a bacterium which causes bartonellosis in residents of high altitude valleys of the Andes, were identified by immunoblot and immunoprecipitation using rabbit anti-Bartonella sera as well as sera of patients. The antigens were designated according to their relative molecular mass which ranged from 16 to 160 kDa. Twelve antigens were detected by antibodies in sera of bartonellosis patients using immunoblot, of which six antigens were detected by immunoprecipitation. Antigens 25, 46, 65, 75, 99, and 160 were identified as probable cell wall antigens. Antigens 50, 65, and 75 detected long-persisting antibodies. Crude Bartonella antigen applied to ELISA reacted with anti-Chlamydia psittaci antibody as well as with antibody of unknown identity in human sera, whereas immunoblot and immunoprecipitation with Triton soluble antigens revealed Bartonella-specific results. Seven Bartonella antigens were prepared by high performance liquid chromatography of which one antigen (48 kDa) reacted Bartonella-specific when applied to ELISA. It was concluded that specificity of antibody determination with crude Bartonella antigen should be confirmed by either immunoblot or immunoprecipitation.
利用兔抗巴尔通体血清以及患者血清，通过免疫印迹法和免疫沉淀法鉴定出了24种巴尔通体杆菌抗原，这种细菌可在安第斯山脉高海拔山谷的居民中引发巴尔通体病。这些抗原根据其相对分子质量命名，范围在16至160 kDa之间。使用免疫印迹法在巴尔通体病患者血清中检测到12种抗原，其中6种抗原通过免疫沉淀法检测到。抗原25、46、65、75、99和160被确定为可能的细胞壁抗原。抗原50、65和75检测到持续存在的抗体。应用于酶联免疫吸附测定（ELISA）的粗制巴尔通体抗原与抗鹦鹉热衣原体抗体以及人血清中身份不明的抗体发生反应，而用Triton可溶性抗原进行的免疫印迹法和免疫沉淀法显示出巴尔通体特异性结果。通过高效液相色谱法制备了7种巴尔通体抗原，其中一种抗原（48 kDa）应用于ELISA时呈现出巴尔通体特异性反应。得出的结论是，使用粗制巴尔通体抗原进行抗体测定的特异性应通过免疫印迹法或免疫沉淀法加以确认。
