Knobloch J, Solano L, Alvarez O, Delgado E
Trop Med Parasitol. 1985 Dec;36(4):183-5.
Two strains of Bartonella bacilliformis were cultured on Columbia agar supplemented with 5% defibrinated human blood. Antigens prepared from these cultures were used for determination of antibodies by fluorescence antibody test (FAT) indirect haemagglutination (IHA) and an enzyme immunoassay (ELISA). One hundred and eighty-seven human sera from B. bacilliformis-endemic areas of Peru were tested of which 63.6% were reactive. ELISA was the most sensitive test, followed by FAT and IHA. IgM antibody was determined by FAT with the IgM fraction of test sera. It was present not only in patients with Oroya fever but also in some healthy individuals as well as in one patient with chronic bartonellosis (verruga peruana). Substantial differences in antigenic activity between the two strains of B. bacilliformis were not observed. Cross reactivity with sera containing antibodies to bacteria other than B. bacilliformis was not noted. For identification of Oroya fever patients in the field, an eosin/thiazine stain of blood smears was found to be appropriate.
将两株杆菌状巴尔通体在添加5%去纤维蛋白人血的哥伦比亚琼脂上培养。从这些培养物中制备的抗原用于通过荧光抗体试验（FAT）、间接血凝试验（IHA）和酶免疫测定（ELISA）来测定抗体。检测了来自秘鲁杆菌状巴尔通体流行地区的187份人血清，其中63.6%呈反应性。ELISA是最敏感的检测方法，其次是FAT和IHA。通过用检测血清的IgM组分进行FAT来测定IgM抗体。它不仅存在于奥罗亚热患者中，也存在于一些健康个体以及一名慢性巴尔通体病（秘鲁疣）患者中。未观察到两株杆菌状巴尔通体之间的抗原活性存在实质性差异。未发现与含有除杆菌状巴尔通体以外细菌抗体的血清有交叉反应。对于现场奥罗亚热患者的诊断，发现血液涂片的伊红/噻嗪染色是合适的。
