Olofsson Karl, Hammarström Björn, Wiklund Martin
Department of Applied Physics, KTH Royal Institute of Technology, SE-106 91 Stockholm, Sweden.
Micromachines (Basel). 2018 Nov 14;9(11):594. doi: 10.3390/mi9110594.
Systems and devices for in vitro tissue modelling and engineering are valuable tools, which combine the strength between the controlled laboratory environment and the complex tissue organization and environment in vivo. Device-based tissue engineering is also a possible avenue for future explant culture in regenerative medicine. The most fundamental requirements on platforms intended for tissue modelling and engineering are their ability to shape and maintain cell aggregates over long-term culture. An emerging technology for tissue shaping and culture is ultrasonic standing wave (USW) particle manipulation, which offers label-free and gentle positioning and aggregation of cells. The pressure nodes defined by the USW, where cells are trapped in most cases, are stable over time and can be both static and dynamic depending on actuation schemes. In this review article, we highlight the potential of USW cell manipulation as a tool for tissue modelling and engineering.
用于体外组织建模和工程的系统和设备是有价值的工具，它们将受控实验室环境的优势与体内复杂的组织结构和环境相结合。基于设备的组织工程也是再生医学中未来外植体培养的一条可能途径。对用于组织建模和工程的平台的最基本要求是它们在长期培养中塑造和维持细胞聚集体的能力。一种新兴的组织塑造和培养技术是超声驻波（USW）粒子操纵，它提供无标记且温和的细胞定位和聚集。由USW定义的压力节点，在大多数情况下细胞被困在其中，随时间是稳定的，并且根据驱动方案可以是静态的和动态的。在这篇综述文章中，我们强调了USW细胞操纵作为组织建模和工程工具的潜力。
